{"title":"细胞外聚羟基烷酸酯(PHA)解聚合酶:PHA降解的关键酶","authors":"D. Jendrossek","doi":"10.1002/3527600035.BPOL3B03","DOIUrl":null,"url":null,"abstract":"Introduction \nHistorical Outline \nIdentification and Isolation of Extracellular d-Poly(HA)-Degrading Microorganisms \nCharacterization of Poly(HA)-Degrading Microorganisms \nBiochemical Properties of Extracellular d-Poly(HA) Depolymerases \nMolecular Biology and Functional Analysis of d-Poly(3HASCL) Depolymerases \nPhaZ7, a new Type of Thermoalkalophilic Hydrolase of P. lemoignei with high Specificity for Amorphous Poly(HASCL) \nMolecular Biology and Functional Analysis of d-Poly(HAMCL) Depolymerases \nEnantioselectivity and Hydrolysis Products of Poly(HA) Depolymerases \nRegulation of Poly(HA) Depolymerase Synthesis \nInfluence of Physico-chemical Properties of the Polymer on its Biodegradability \nOutlook and Perspectives \nPatents \nAcknowledgments \n \n \nKeywords: \n \nPHA depolymerase; \nPHB depolymerase; \nPHO depolymerase; \nPaucimonas lemoignei; \nPseudomonas lemoignei; \nPseudomonas fluorescens GK13; \npoly(HA); \nnative PHA; \ndenatured PHB; \nartificial PHA; \natactic PHA; \nlipase; \nserine hydrolase; \ncatalytic triad; \nsubstrate-binding domain; \ncarboxyesterase","PeriodicalId":165163,"journal":{"name":"Biopolymers Online","volume":"69 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2002-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"49","resultStr":"{\"title\":\"Extracellular Polyhydroxyalkanoate (PHA) Depolymerases: The Key Enzymes of PHA Degradation\",\"authors\":\"D. Jendrossek\",\"doi\":\"10.1002/3527600035.BPOL3B03\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Introduction \\nHistorical Outline \\nIdentification and Isolation of Extracellular d-Poly(HA)-Degrading Microorganisms \\nCharacterization of Poly(HA)-Degrading Microorganisms \\nBiochemical Properties of Extracellular d-Poly(HA) Depolymerases \\nMolecular Biology and Functional Analysis of d-Poly(3HASCL) Depolymerases \\nPhaZ7, a new Type of Thermoalkalophilic Hydrolase of P. lemoignei with high Specificity for Amorphous Poly(HASCL) \\nMolecular Biology and Functional Analysis of d-Poly(HAMCL) Depolymerases \\nEnantioselectivity and Hydrolysis Products of Poly(HA) Depolymerases \\nRegulation of Poly(HA) Depolymerase Synthesis \\nInfluence of Physico-chemical Properties of the Polymer on its Biodegradability \\nOutlook and Perspectives \\nPatents \\nAcknowledgments \\n \\n \\nKeywords: \\n \\nPHA depolymerase; \\nPHB depolymerase; \\nPHO depolymerase; \\nPaucimonas lemoignei; \\nPseudomonas lemoignei; \\nPseudomonas fluorescens GK13; \\npoly(HA); \\nnative PHA; \\ndenatured PHB; \\nartificial PHA; \\natactic PHA; \\nlipase; \\nserine hydrolase; \\ncatalytic triad; \\nsubstrate-binding domain; \\ncarboxyesterase\",\"PeriodicalId\":165163,\"journal\":{\"name\":\"Biopolymers Online\",\"volume\":\"69 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2002-12-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"49\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biopolymers Online\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1002/3527600035.BPOL3B03\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biopolymers Online","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/3527600035.BPOL3B03","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Extracellular Polyhydroxyalkanoate (PHA) Depolymerases: The Key Enzymes of PHA Degradation
Introduction
Historical Outline
Identification and Isolation of Extracellular d-Poly(HA)-Degrading Microorganisms
Characterization of Poly(HA)-Degrading Microorganisms
Biochemical Properties of Extracellular d-Poly(HA) Depolymerases
Molecular Biology and Functional Analysis of d-Poly(3HASCL) Depolymerases
PhaZ7, a new Type of Thermoalkalophilic Hydrolase of P. lemoignei with high Specificity for Amorphous Poly(HASCL)
Molecular Biology and Functional Analysis of d-Poly(HAMCL) Depolymerases
Enantioselectivity and Hydrolysis Products of Poly(HA) Depolymerases
Regulation of Poly(HA) Depolymerase Synthesis
Influence of Physico-chemical Properties of the Polymer on its Biodegradability
Outlook and Perspectives
Patents
Acknowledgments
Keywords:
PHA depolymerase;
PHB depolymerase;
PHO depolymerase;
Paucimonas lemoignei;
Pseudomonas lemoignei;
Pseudomonas fluorescens GK13;
poly(HA);
native PHA;
denatured PHB;
artificial PHA;
atactic PHA;
lipase;
serine hydrolase;
catalytic triad;
substrate-binding domain;
carboxyesterase