理化应激源作用下鱼类中毒白细胞指标及反应性的预测价值

L. Karpenko, I. Makhnin, Yu. Berenev, A. Bakhta
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引用次数: 0

摘要

目的:探讨几种物化应激源作用下鱼类中毒白细胞指标及反应性的预后价值。材料和方法。研究的对象是普通Rutilus Rutilus (L.)的蟑螂夫人。参照类似物原则分为对照组和试验组(n = 90)。平均质量(PP) 5.29±1.0 g,体长(TL) - 7.22±0.39 cm,为幼鱼发育时期(性器官未安装)。在水族箱(体积- 200 l)中积累个体(10天),并包含以下指标。我们检查了核心指数、中毒白细胞指数、血白细胞剪切指数和白细胞指数。作为物理化学应激源,研究了铜的皮下浓度(在0.01 mg/l和0.1 mg/l铜盐中暴露7 d和14 d);地磁风暴主期和初始期的模拟;高温作用。结果。对中毒白细胞指数和反应性的分析表明,克雷布斯指数和a -白细胞指数对鱼的预后最重要。白细胞剪切指数和白细胞中毒指数的信息含量存在争议。血白细胞移位指数仅随铜盐暴露14 d和物理应激源的作用而增加。在暴露于铜盐7天期间,血液白细胞移位指数没有统计学上的显著变化,这可能表明该指数的敏感性较低。计算中毒白细胞指数时得到的结果与测定克雷布斯指数时得到的结果相似。该指标在物理化学应激源作用下的具体变化尚未确定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Prognostic value of some leukocyte indices of intoxication and reactivity in fish under the action of physico-chemical stressors
Purpose: determining the prognostic value of leukocyte indexes of intoxication and reactivity in fish under the action of some physicochemical stressors.Materials and methods. The object of the study was the lady of the roach of ordinary Rutilus Rutilus (L.). Control and experimental groups were formed taking into account the principle of analogues (n = 90). The average mass (PP) is 5.29 ± 1.0 g, length (TL) - 7.22 ± 0.39 cm, fish of the juvenile period of development (the sexual structure was not installed). The individuals were accumulated (10 days) and contained the following indices in aquariums (volume - 200 l.) We examined the core index, a leukocytic index of intoxication, blood leukocyte shear index, and a leukocytal index. As physico-chemical stressors, subcutal concentrations of copper (exposition in copper salt 0.01 mg/l and 0.1 mg/l for 7 and 14 days) were studied; imitation of the main phase and the initial period of the phase of geomagnetic storm; The action of high temperature.Results. The analysis of leukocyte indexes of intoxication and reactivity showed that in fish the Krebs index and a leukocytic index have the greatest prognostic importance. The information content of the blood leukocyte shear index and the leukocyte intoxication index should be considered controversial. The blood leukocyte shift index increased only with the exposure of copper salts for 14 days and with the action of physical stressors. Statistically significant changes in the blood leukocyte shift index during exposure by copper salts for 7 days have not been revealed, which may indicate a low sensitivity of the index. The results obtained during the calculation of the leukocyte index of intoxication are similar to the results when determining the Krebs index. Specific changes in the index under the action of physico-chemical stressors have not been identified.
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