{"title":"动物组织中碘甲状腺原氨酸不同提取方法的比较。","authors":"E Krysin","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Four different methods for thyroid hormones concentration in various tissues have been compared. The methods involve homogenization, extraction with 95% ethanol and assay of iodothyronines by a highly sensitive RIA. The method described by Chopra et al. has been selected and used for further estimations as the most reliable and convenient for a continuous laboratory work. The T4 levels (means +/- SE) found in the liver, kidney and skeletal muscle were 22.49 +/- 2.19, 14.89 +/- 1.56 and 8.33 +/- 1.73 ng per g wet tissue. The T3 contents were 8.53 +/- 1.08, 9.52 +/- 0.69 and 4.90 +/- 0.76 ng per g wet tissue, respectively.</p>","PeriodicalId":20364,"journal":{"name":"Polskie archiwum weterynaryjne","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparison of different methods for iodothyronines extraction from animal tissues.\",\"authors\":\"E Krysin\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Four different methods for thyroid hormones concentration in various tissues have been compared. The methods involve homogenization, extraction with 95% ethanol and assay of iodothyronines by a highly sensitive RIA. The method described by Chopra et al. has been selected and used for further estimations as the most reliable and convenient for a continuous laboratory work. The T4 levels (means +/- SE) found in the liver, kidney and skeletal muscle were 22.49 +/- 2.19, 14.89 +/- 1.56 and 8.33 +/- 1.73 ng per g wet tissue. The T3 contents were 8.53 +/- 1.08, 9.52 +/- 0.69 and 4.90 +/- 0.76 ng per g wet tissue, respectively.</p>\",\"PeriodicalId\":20364,\"journal\":{\"name\":\"Polskie archiwum weterynaryjne\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1990-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Polskie archiwum weterynaryjne\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Polskie archiwum weterynaryjne","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
比较了四种不同的方法测定甲状腺激素在不同组织中的浓度。方法包括均质，95%乙醇提取和高灵敏度RIA测定碘甲状腺原氨酸。Chopra等人描述的方法已被选择并用于进一步估计，因为它对于连续的实验室工作来说是最可靠和方便的。肝脏、肾脏和骨骼肌的T4水平(平均+/- SE)分别为22.49 +/- 2.19、14.89 +/- 1.56和8.33 +/- 1.73 ng / g湿组织。T3含量分别为8.53 +/- 1.08、9.52 +/- 0.69和4.90 +/- 0.76 ng / g湿组织。
Comparison of different methods for iodothyronines extraction from animal tissues.
Four different methods for thyroid hormones concentration in various tissues have been compared. The methods involve homogenization, extraction with 95% ethanol and assay of iodothyronines by a highly sensitive RIA. The method described by Chopra et al. has been selected and used for further estimations as the most reliable and convenient for a continuous laboratory work. The T4 levels (means +/- SE) found in the liver, kidney and skeletal muscle were 22.49 +/- 2.19, 14.89 +/- 1.56 and 8.33 +/- 1.73 ng per g wet tissue. The T3 contents were 8.53 +/- 1.08, 9.52 +/- 0.69 and 4.90 +/- 0.76 ng per g wet tissue, respectively.
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