玉米(Zea mays)双单倍体的传统方法研究

Q1 Agricultural and Biological Sciences

Current protocols in plant biology Pub Date : 2017-06-21 DOI:10.1002/cppb.20050

Kimberly Vanous, Adam Vanous, Ursula K. Frei, Thomas Lübberstedt

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引用次数: 19

摘要

商业玉米杂交生产证实了生产自交系的有效性;然而，在育种计划中，新品种的交付一直是一个主要的时间限制。发展自交系的传统方法通常需要6至10代的自花授粉才能获得足够的纯合子。为了避免与自交系开发相关的时间和成本，双单倍体(DH)系统已广泛应用于玉米的商业生产。仅在两代内，DH系统就能产生完全纯合的同质系。本文描述了一个典型的玉米DH系统，利用花青素标记R1-nj或Pl1进行单倍体选择。©2017 by John Wiley &儿子,Inc。

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Generation of Maize (Zea mays) Doubled Haploids via Traditional Methods

Commercial maize hybrid production has corroborated the usefulness of producing inbred lines; however, the delivery of new lines has always been a major time constraint in breeding programs. Traditional methods for developing inbred lines typically require 6 to 10 generations of self-pollination to obtain sufficient homozygosity. To bypass the time and costs associated with the development of inbred lines, doubled haploid (DH) systems have been widely adopted in the commercial production of maize. Within just two generations, DH systems can create completely homozygous and homogeneous lines. A typical maize DH system, utilizing anthocyanin markers R1-nj or Pl1 for haploid selection, is described in this protocol. © 2017 by John Wiley & Sons, Inc.

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来源期刊

Current protocols in plant biology Agricultural and Biological Sciences-Plant Science

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. Current Protocols in Plant Biology provides reproducible step-by-step instructions for protocols relevant to plant research. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols in Plant Biology to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Plant Biology is the comprehensive source for protocols in the multidisciplinary field of plant biology, providing an extensive range of protocols from basic to cutting edge. Coverage includes: Extraction and analysis of DNA, RNA, proteins Chromosome analysis Transcriptional analysis Protein expression Metabolites Plant enzymology Epigenetics Plant genetic transformation Mutagenesis Arabidopsis, Maize, Poplar, Rice, and Soybean, and more.