{"title":"大鼠垂体雌激素和促性腺激素的同时放射自显影和免疫组织化学定位。","authors":"D. Keefer, W. Stumpf, P. Petrusz, M. Sar","doi":"10.1097/00006254-197508000-00016","DOIUrl":null,"url":null,"abstract":"A combined autoradiographic and immunohistochemical technique is introduced which is suitable to simultaneously localize hypophyseal sites of nuclear (3-H)-estradiol uptake and anti-hCG-characterized \"gonadotropes\". Most, but not all, \"gonadotropes\" show significant nuclear concentration of radioactivity. A large percentage of non-\"gonadotropes\" also show nuclear uptake of label.","PeriodicalId":101557,"journal":{"name":"The American journal of anatomy","volume":"79 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"14","resultStr":"{\"title\":\"Simultaneous autoradiographic and immunohistochemical localization of estrogen and gonadotropin in the rat pituitary.\",\"authors\":\"D. Keefer, W. Stumpf, P. Petrusz, M. Sar\",\"doi\":\"10.1097/00006254-197508000-00016\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A combined autoradiographic and immunohistochemical technique is introduced which is suitable to simultaneously localize hypophyseal sites of nuclear (3-H)-estradiol uptake and anti-hCG-characterized \\\"gonadotropes\\\". Most, but not all, \\\"gonadotropes\\\" show significant nuclear concentration of radioactivity. A large percentage of non-\\\"gonadotropes\\\" also show nuclear uptake of label.\",\"PeriodicalId\":101557,\"journal\":{\"name\":\"The American journal of anatomy\",\"volume\":\"79 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1900-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"14\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The American journal of anatomy\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1097/00006254-197508000-00016\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The American journal of anatomy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1097/00006254-197508000-00016","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Simultaneous autoradiographic and immunohistochemical localization of estrogen and gonadotropin in the rat pituitary.
A combined autoradiographic and immunohistochemical technique is introduced which is suitable to simultaneously localize hypophyseal sites of nuclear (3-H)-estradiol uptake and anti-hCG-characterized "gonadotropes". Most, but not all, "gonadotropes" show significant nuclear concentration of radioactivity. A large percentage of non-"gonadotropes" also show nuclear uptake of label.
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