{"title":"[酶免疫法测定儿童尿中分泌性免疫球蛋白A]。","authors":"W Kunze, M Seyfarth","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The estimation of secretory immunoglobulin A (sIgA) by an enzyme immunoassay (EIA) was used as heterogenic solid-phase assay. A monoclonal antibody directed against the human secretoric part and a peroxidase-marked anti-IgA serum may mediate the reaction. The test was evaluated on urine of 59 healthy children aged from 0-14 years. An age-dependent increase of sIgA concentration (mg/l) and excretion up to the 6th months of life was found. Thereafter the concentration did reach a constant value of 0.5 mg/l sIgA and 0.4 mg sIgA/g creatinine, respectively. The normal values may follow a logarithmic distribution. The presented method is highly sensitive, simple and safe.</p>","PeriodicalId":76863,"journal":{"name":"Zeitschrift fur Urologie und Nephrologie","volume":"83 11","pages":"617-24"},"PeriodicalIF":0.0000,"publicationDate":"1990-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Determination of secretory immunoglobulin A in urine of children with enzyme immunoassay].\",\"authors\":\"W Kunze, M Seyfarth\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The estimation of secretory immunoglobulin A (sIgA) by an enzyme immunoassay (EIA) was used as heterogenic solid-phase assay. A monoclonal antibody directed against the human secretoric part and a peroxidase-marked anti-IgA serum may mediate the reaction. The test was evaluated on urine of 59 healthy children aged from 0-14 years. An age-dependent increase of sIgA concentration (mg/l) and excretion up to the 6th months of life was found. Thereafter the concentration did reach a constant value of 0.5 mg/l sIgA and 0.4 mg sIgA/g creatinine, respectively. The normal values may follow a logarithmic distribution. The presented method is highly sensitive, simple and safe.</p>\",\"PeriodicalId\":76863,\"journal\":{\"name\":\"Zeitschrift fur Urologie und Nephrologie\",\"volume\":\"83 11\",\"pages\":\"617-24\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1990-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zeitschrift fur Urologie und Nephrologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zeitschrift fur Urologie und Nephrologie","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Determination of secretory immunoglobulin A in urine of children with enzyme immunoassay].
The estimation of secretory immunoglobulin A (sIgA) by an enzyme immunoassay (EIA) was used as heterogenic solid-phase assay. A monoclonal antibody directed against the human secretoric part and a peroxidase-marked anti-IgA serum may mediate the reaction. The test was evaluated on urine of 59 healthy children aged from 0-14 years. An age-dependent increase of sIgA concentration (mg/l) and excretion up to the 6th months of life was found. Thereafter the concentration did reach a constant value of 0.5 mg/l sIgA and 0.4 mg sIgA/g creatinine, respectively. The normal values may follow a logarithmic distribution. The presented method is highly sensitive, simple and safe.
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