[体外实验中pH、温度和白蛋白含量对胎鼠肝细胞活力的影响]。

Zeitschrift fur experimentelle Chirurgie, Transplantation, und kunstliche Organe : Organ der Sektion Experimentelle Chirurgie der Gesellschaft fur Chirurgie der DDR Pub Date : 1990-01-01

C Hellmann, U Otting, P Grossmann

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摘要

体外实验考察了培养液pH、温度和白蛋白含量对培养肝细胞活力的影响，并通过台盼蓝排斥试验考察了其影响。培养液pH值在6.8 ~ 7.4之间变化，对肝细胞活力和台盼蓝排除试验均无影响。在初始pH值为7.8的孵育培养基中，出现了活力降低的结果。在培养温度为4℃的情况下，生命染料排除试验是无用的，因为关于肝细胞活力的结果只是明显更好。添加人血清白蛋白对于肝细胞在孵育期间的良好存活是必要的。

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[The effect of pH, temperature and albumin content on the vitality of fetal rat liver cells during in vitro tests].

The influence of pH, temperature, and albumin content in the incubation medium on the vitality incubated hepatocytes was tested in vitro as well as the effects in the trypan-blue exclusion test. pH changes between 6.8 and 7.4 in the incubation medium have not any influence on the vitality of the hepatocytes or the trypan-blue exclusion test. Reduced vitality results occurred in an initial pH 7.8 in the incubation medium. The vital dye exclusion test is not useful in case of incubation temperature of 4 degrees C, since the results regarding the vitality of the hepatocytes are only apparently better. An addition of human serum albumin is necessary for a good survival of the hepatocytes during the incubation.

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Zeitschrift fur experimentelle Chirurgie, Transplantation, und kunstliche Organe : Organ der Sektion Experimentelle Chirurgie der Gesellschaft fur Chirurgie der DDR

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