DEAE亲和凝胶蓝纯化转铁蛋白和乳铁蛋白。

M C Chung, S L Chan, S Shimizu
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引用次数: 0

摘要

1. 介绍了一种简单的转铁蛋白和乳铁蛋白的纯化方法。2. 该方法包括染料配体色谱的初步步骤,使用DEAE Affi-Gel Blue作为凝胶基质,pH为7.5。在这个色谱步骤中,转铁蛋白和乳铁蛋白很容易通过开始缓冲洗脱从其他蛋白质中分离出来。3.各种血清转铁蛋白(猴、人、兔、猪、鸡和鸭)和卵转铁蛋白在DEAE Affi-Gel Blue色谱上的色谱行为差异可归因于转铁蛋白在0.02 M磷酸钾缓冲液(pH 7.5)中阴离子电荷的差异,从而导致凝胶基质对这些蛋白质分子的不同阻滞。4. 人乳铁蛋白的DEAE Affi-Gel Blue色谱结果与转铁蛋白不同。这可能反映了乳铁蛋白和转铁蛋白与凝胶基质之间相互作用强度的差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Purification of transferrins and lactoferrin using DEAE affi-gel blue.

1. A simple method for purifying transferrins and lactoferrin is described. 2. The method consists of a preliminary step of dye-ligand chromatography using DEAE Affi-Gel Blue as the gel matrix at pH 7.5. In this chromatographic step, the transferrins and lactoferrin were readily separated from the bulk of the other proteins by start buffer elution. 3. Differences in the chromatographic behaviour of the various serum transferrins (monkey, human, rabbit, pig, chicken and duck) and ovotransferrin upon DEAE Affi-Gel Blue chromatography can be attributed to differences in the anionic charge of the transferrins in 0.02 M potassium phosphate buffer, pH 7.5, thus resulting in the differential retardation of these protein molecules by the gel matrix. 4. The result of DEAE Affi-Gel Blue chromatography of human lactoferrin is different from that for the transferrins. This may possibly reflect the differences in the strength of interaction between lactoferrin and transferrin with this gel matrix.

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