GENETIC DIFFERENTIATION IN A COLLECTION OF CUCUMEROPSIS MANNII NAUDIN (CUCURBITACEAE) FROM CÔTE D’IVOIRE BY USING ISSR MARKERS

Thirty accessions from three cultivars (small seeds, medium seeds and large seeds), collected from center, east, north and south of Côte d'Ivoire, were analyzed for genetic differentiation. Five primers (Sola1, Sola4, Sola7, Sola8 and B4) were used to identify ISSR markers in C. mannii. These primers revealed a total of 55 bands of which 52 bands (93.06 %) are polymorphic. Molecular Analysis of Variance (AMOVA) indicates a weak genetic differentiation of the three cultivars (Fst = 0.048 or 5 %; p = 0.01). When considering the collection area, a weak differentiation between accessions was observed (Fst = 0.027 or 3 %; p = 0.01). On the other hand, a strong genetic differentiation was observed between accessions of the same cultivar (Fst = 0.268 or 24 %; p = 0.01) and between individuals of the same accession, for which a highly significant differentiation (Fst = 0.261 or 74 %; p = 0.01) was observed. For the constitution of a gene bank, it is necessary to keep many individuals in a limited number of accessions from few regions. Indeed, the dendrogram of C. mannii indicates a weak differentiation between cultivars and therefore their belonging to the same common ancestor.