J. Ferrer Ortas, P. Mahou, S. Escot, C. Stringari, Nicolas B. David, Laure Bally-Cuif, Nicolas Dray, Michel Négrerie, Willy Supatto, E. Beaurepaire
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Color TSFG microscopy of red blood cells and oxygenation
We present color third-order sum-frequency generation (color TSFG) microscopy, a multiphoton imaging strategy based on the simultaneous detection of several third-order coherent signals produced by two synchronized femtosecond pulse trains. We demonstrate that it can be used to obtain red blood cell (RBC)-specific label-free contrast in live zebrafish and is a promising tool for probing RBC oxygenation.
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