Metabolic and functional state of the liver at the combined effect of lead and fluorine on the background of bioprotectors application

Introduction. The potential danger of combined effect of lead and fluorine to human health motivates the search and experimental substantiation of physiologically acceptable ways of bioprophylaxis directed to preventing or reducing the negative effect on the organism. Objective: the study of the dynamics of the metabolic and functional state of the liver under conditions of combined effect of lead and fluorine without application of bioprotectors and on the background of bioprotectors application. Material and Methods. 4 series of researches were carried out on white rats, each according to the orthogonal planning scheme 22. Lead and fluorine were administered by intragastric within 30 days: in the first series — without bioprotectors application, in the second series — on the background of pectin application, in the third series — pectin and calcium application, in the fourth series — the complex of pectin, calcium and triovit application. The level of diene conjugates, the active products of thiobarbituric acid, the activities of superoxide dis-mutase and catalase, the index of general antioxidant activity in the liver tissue, the activities of alanine aminotransferases, aspartate aminotransferases and alkaline phosphatase in blood serum, concentration of urea were determined at the 15th and 30th day of the experiments, the integral coefficient of antioxidant defense and the type of combined action were calculated. Results. The combined effect of lead and fluorine leads to an imbalance between the indicators of lipoperox-idation and antioxidant activity of the liver tissue, the increased activities of alanine aminotransferases and aspartate aminotransferases, the phase changes in alkaline phosphatase activity, and urea concentration in serum. The application of pectin did not affect the indicators. Pectin and calcium have improved the mechanisms of antioxidant system and the activity of enzymes. The complex of pectin, calcium and triovit normalizes metabolic processes in the liver tissue and improves its functional state. The type of the combined action depends on the registered effect, the duration of the stress factor and the applied bioprotektor. Conclusion. The optimal prophylactic effect under the combined action of lead and fluorine is achieved with the help of a complex of pectin, calcium and triovit. Key Words: lead, fluorine, combined effect, pectin, calcium, triovit, indicators of lipid peroxidation, antioxidant defense, enzyme activity.