石蜡切片原位杂交法检测宫颈癌组织中HPV-16 DNA。

Proceedings of the Chinese Academy of Medical Sciences and the Peking Union Medical College = Chung-kuo i hsueh k'o hsueh yuan, Chung-kuo hsieh ho i k'o ta hsueh hsueh pao Pub Date : 1990-01-01

W Zhang, Y Sun, M Shang, S Jin, X Liang, K Liang, A Wu, G Nan, X Wang, W Zhang

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引用次数: 0

摘要

用3h - dctp标记的HPV-16 DNA石蜡切片原位杂交探针和32p - dctp标记探针点杂交(Tm-17)检测了38例宫颈癌和1例宫颈尖锐湿疣患者的人乳头瘤病毒(HPV) 16型DNA。结果表明，石蜡切片原位杂交法检测HPV-16 DNA阳性率为74.4%(29/39)，斑点杂交法检测阳性率为71.8%(28/29)。两种方法间无显著差异。因此，我们证实石蜡切片原位杂交是宫颈HPV感染诊断的一种信息丰富、可靠和敏感的方法。

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Detection of HPV-16 DNA in cervical carcinoma by paraffin section in situ hybridization.

Thirty-eight cervical carcinomas and 1 cervical condyloma were examined for the presence of human papillomavirus (HPV) type 16 DNA using 3H-dCTP-labelled HPV-16 DNA probe in paraffin section in situ hybridization and 32P-dCTP-labelled probe in dot blot hybridization (Tm-17). The results showed an HPV-16 DNA positive rate in our series of about 74.4% (29/39) as determined by paraffin section in situ hybridization and about 71.8% (28/29) by dot blot hybridization. There was no significant difference between the two methods. We thus confirmed that paraffin section in situ hybridization is an informative, reliable, and sensitive method for the diagnosis of cervical HPV infection.

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Proceedings of the Chinese Academy of Medical Sciences and the Peking Union Medical College = Chung-kuo i hsueh k'o hsueh yuan, Chung-kuo hsieh ho i k'o ta hsueh hsueh pao

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