犬疱疹病毒日本分离株的限制性内切酶分析。

X N Xuan, T Horimoto, M Ono, J A Limcumpao, Y Tohya, M Azetaka, E Takahashi, T Mikami
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引用次数: 8

摘要

对日本18株犬疱疹病毒(CHV)分离株和2株参比株进行了限制性内切酶分析。为了选择适合CHV分离株分化的限制性内切酶,我们使用了10种酶,发现HindIII、XbaI和PvuII 3种酶对菌株分化有帮助。利用这些酶,来自无亲缘关系个体的CHV分离物很容易相互分化。相比之下,来自同一凋落物的所有分离株都不能根据限制性解理模式进行区分。但在同一凋落物或同一个体的分离株之间存在轻微的移动性变化。结果表明,该方法为CHV感染流行病学调查提供了有力的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Restriction endonuclease analysis of canine herpesviruses isolated in Japan.

Eighteen canine herpesvirus (CHV) isolates from Japan and two reference strains were compared by restriction endonuclease analysis technique using total DNA extracts from cells infected with the viruses. In order to select the suitable restriction endonucleases for differentiation of CHV isolates, ten enzymes were used and three of them, HindIII, XbaI, and PvuII, were found to be useful for strain differentiation. With these enzymes, CHV isolates from unrelated individuals were readily differentiated from each other. In contrast, all the isolates derived from the same litter were not distinguishable on the basis of restriction cleavage patterns. However, slight mobility shifts were observed among the isolates from the same litter or the same individual. The results showed that this method provides a powerful tool for epidemiological surveys of CHV infection.

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