罗勒(Ocimum baslicum L.)和芫荽(Coriandrum satirum L.)油提取物对变形链球菌抑菌活性的体外评价

M. Lourenço, A. C. Bernardi, Nádia Lunardi, Rodolfo Jorge Noeck Neto, P. M. Bernardi, E. M. Boeck
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引用次数: 2

摘要

植物提取物和精油对多种微生物(包括丝状真菌、酵母菌和细菌)的生长具有控制作用。为了评价植物提取物的抑菌活性,有必要确定抑制微生物生长的较低物质量。这个值被称为最小抑制浓度(MIC)。本研究旨在验证罗勒(Ocimum basilicum L.)和芫荽(Coriandrum sativum L.)油提取物对变形链球菌(ATCC 25175)的抑菌作用和最低抑菌浓度(MIC)。抗菌活性测定采用微量稀释法,并按照CLSI(以前称为NCCLS)、细菌标准M7-A6 (NCCLS, 2003)和标准M27-A2 (NCCLS, 2002)的建议进行。所有的实验都是三次重复进行的。结果表明,用微量稀释法测定肉汤中的最低抑菌浓度(MIC)显示,罗勒和芫荽提取物精油对变形链球菌具有抑菌活性。1:4浓度的罗勒是抑菌剂,1:3浓度的罗勒是杀菌剂。1:2浓度的香菜是抑菌剂,1:1浓度的香菜是杀菌剂。结果表明,罗勒对香菜的抑菌活性较高。我们还观察到,提取物稀释度越大,其有效性越低。o评估牙科专业人员对使用先进放射技术的态度和做法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vitro evaluation of the antimicrobial activity of Basil (Ocimum baslicum L.) and Coriander (Coriandrum satirum L.) oil extracts on Streptococus mutans
Plant extracts and essential oils show efficiency on growth control in a wide variation of microorganisms, including filamentous fungi, yeasts and bacteria. To evaluate antimicrobial activity of plant extracts, determine the lower quantity of substance to inhibit the microorganism test growth is necessary. This value is known as Minimum Inhibitory Concentration (MIC). This study had as aim to verify the antimicrobial action and the Minimum Inhibitory Concentration (MIC) of basil (Ocimum basilicum L.) and coriander (Coriandrum sativum L.) oil extract before S. mutans (ATCC 25175) strains. Antimicrobial activity determination was carried out by microdilution method and performed according to recommendations of CLSI (previously known as NCCLS), standard M7-A6 (NCCLS, 2003) for bacteria, and standard M27-A2 (NCCLS, 2002). All the experiments were carried out in triplicate. Results showed the Minimum Inhibitory Concentration (MIC) determination by microdilution method in broth showed Ocimum basilicum L. and Coriandrum sativum L. extract oils presented inhibitory activity before S. mutans strain. Basil in 1:4 concentration is bacteriostatic and in 1:3 concentration is bactericide. Coriander in 1:2 concentration is bacteriostatic and in 1:1 concentration is bactericide. We concluded that basil presented higher inhibitory activity regarding to the coriander. We also observed as bigger the extract dilution, lower their effectivity.o assess the attitude and practice of dental professionals towards using of advance Radiographic technique.
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