全内反射荧光(TIRF)显微镜在细胞生物成像中的应用

2007 Asia Optical Fiber Communication and Optoelectronics Conference Pub Date : 2007-12-26 DOI:10.1109/AOE.2007.4410884

L. Jin, R. Lee, S. K. Kong, W. Yuan, H. Ho, Chinlon Lin

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引用次数: 3

摘要

瞬变场成像和传感器技术已经广泛应用了很长时间，但直到最近才将红外射频技术引入生物成像。本文将讨论其原因，并简要总结TIRF的最新发展，该技术也是基于倏逝波激发，非常适合于具有异常高信噪比和图像质量的细胞-衬底区域的超薄光学切片(数百纳米)。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Applications of Total Internal Reflection Fluorescence (TIRF) microscopy in cellular bio-imaging

Evanescent field based imaging and sensor technologies have been widely used for a long time, but only recently has TIRF been introduced for bio-imaging. This paper will discuss the reasons and give a brief summary of the recent development of TIRF which is also based on evanescent wave excitation, and which is well suited for ultra thin optical sectioning (hundreds of nanometres) at cell-substrate regions with an unusually high signal-to-noise ratio and image quality.

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2007 Asia Optical Fiber Communication and Optoelectronics Conference

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