C. Malta, Eskálath Morganna Silva Ferreira, C. M. Coelho, R. S. Pimenta
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引用次数: 2

摘要

拮抗微生物(细菌、酵母菌和丝状真菌)能够对各种病原菌发挥拮抗作用,用于防治水果和蔬菜的各种病害。本研究旨在分离和检测产自益生菌DC的病原菌对寄生曲霉、炭疽菌和果糖念珠菌的抑菌作用。本研究采集了30株cagaiteira的茎叶。根据Rose等人(2010年)的建议,将样品提交表面消毒。片段接种于琼脂马铃薯培养皿25-28℃,接种60天。获得了263株内生真菌,对其进行了纯化,并对其产生抑制性、挥发性或扩散性物质的潜力进行了评估。96个分离株对植物病原菌具有拮抗活性。45株分离物能抑制寄生蜂的生长。68株内生菌对病原菌gloeosporioides具有拮抗活性。对病原菌果支霉只有17种真菌有抑制作用。这些分离株显示有可能产生对抗这些病原体的生物活性物质。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Endophytic fungi Eugenia dysenterica DC as biocontrol of plant pathogens in vitro / Fungos endofíticos de Eugenia dysenterica DC como biocontroladores de fitopatógenos in vitro.
The antagonistic microorganisms (bacteria, yeasts and filamentous fungi) are able to exert an antagonistic effect on various pathogens being used to control various diseases of fruits and vegetables. This study aimed to isolate and test microorganisms present in Eugenia dysenterica DC against Aspergillus parasiticus, Colletotrichum gloeosporioides, and Monilinia fructicola and in vitro. Stems and leaves were collected from 30 specimens of cagaiteira. The samples were submited to a surface disinfection, according to the proposed by Rose, et. al, (2010). Fragments were inoculated on Petri plates containing the agar Potato 25-28 ° C for 60 days. 263 endophytic fungi strains were obtained, purified and assessed for their potential in to produce inhibitore volatile or diffusible substances. A total of 96 isolates showed antagonistic activity against plant pathogens. 45 isolates being able to inhibit the growth of A. parasiticus. Against the pathogen C. gloeosporioides 68 endophytes showed antagonist activity. Against the pathogen M. fructicola only 17 fungi were able to exert inhibitory effect. These isolates showed potential for the production of bioactive substances against these pathogens.
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