cupuaparru汁制备的开菲尔生物膜中微生物表面分布的研究

Matheus Antonio Nery Ferraro, E. P. Pinto, R. Matos
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引用次数: 17

摘要

近年来,生物材料领域的许多研究都集中在寻找更好的材料作为敷料或临时皮肤替代物,但往往没有成功。开菲尔和库普阿苏在这一领域具有潜在的治疗价值。本研究采用红糖液接种克非尔颗粒制备的克非尔生物膜,对其细菌和酵母的表面分布进行了形态学和统计学分析。获得了6个不同浓度的生物膜样品,在原子力显微镜下对其进行了分析,获得了形貌图像。对表面参数进行统计分析,以确定生物膜中微生物的数量和表面覆盖率。形态学分析表明,生物膜表面由类似细菌和酵母菌的微生物组成。统计分析表明,库普阿苏浓度在10 ~ 40 g之间的生物膜。L -1的细菌数量和覆盖率更高。结果表明,库瓜苏10 g。L -1生物膜是最合适的,因为它具有较高的细菌数量和覆盖率,是一种抗菌剂。从这个角度来看,研究结果表明,生物膜有望在未来的制药工业中得到应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Study of the superficial distribution of microorganisms in kefir biofilms prepared with Cupuaçu juice
Recently, much research in the field of biomaterials has focused on finding better material to serve as a dressing or temporary skin replacement, often without success. Kefir and Cupuacu ( Theobroma grandiflorum Schum) have therapeutic potential for use in this field. In this study, a morphological and statistical analysis of the superficial distribution of bacteria and yeasts in kefir biofilms prepared with Cupuacu juice, obtained from the inoculation of kefir grains in brown sugar solution was performed. Six samples of different concentrations of the biofilms were obtained, which were analyzed in an Atomic Force Microscope to obtain topography images. Statistical analyses were performed on the surface parameters in order to determine the number and surface coverage of microorganisms in biofilms. The morphological analysis showed that the surfaces of the biofilms are composed of microorganisms similar to bacteria and yeasts. Statistical analysis showed that biofilms with a concentration of Cupuacu between 10 and 40 g.L -1 present a greater number and coverage of bacteria. All results show that the Cupuacu 10 g.L -1 biofilm presented the most appropriate, considering that it presented a higher number and coverage of bacteria, which is the antibacterial agent. In this perspective, the results indicate that there is an expectation of biofilms to result in a future application in the pharmaceutical industry.
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