W Pajdak, M Sieradzak-Fleituch, A Dubin, J Owsiński
{"title":"肺部疾病患者支气管肺泡灌洗液中α -1-抗胰蛋白酶和α -1-抗胰蛋白酶-中性粒细胞弹性蛋白酶复合物(初步研究)","authors":"W Pajdak, M Sieradzak-Fleituch, A Dubin, J Owsiński","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>A simple method based on rocket and crossed immunoelectrophoresis was adapted for detection of alpha 1 AT-HNE complexes in BALF: Agarose gel with anti-alpha 1 AT combined with intermediate gel containing anti-HNE was used for detection and evaluation of the ratio of complexes with HNE to the free form of alpha 1 AT. BALF samples from 31 patients divided into 5 groups suffering from various respiratory tract diseases were tested. It was found that 3 out of 8 patients with inflammatory pulmonary diseases showed the presence of both forms of alpha 1 AT, 7 out of 13 patients suffering from cancer showed both forms, whereas the complexed form was not detected in any of 8 patients with asthma bronchiale. It is suggested that the presence of alpha 1 AT-HNE complex may reflect, to some extent, the proteolytic status of pulmonary tissue.</p>","PeriodicalId":7090,"journal":{"name":"Acta medica Hungarica","volume":"48 1-2","pages":"103-9"},"PeriodicalIF":0.0000,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Alpha-1-antitrypsin and alpha-1-antitrypsin-neutrophil elastase complex in bronchoalveolar lavage fluid of patients with pulmonary diseases (pilot study).\",\"authors\":\"W Pajdak, M Sieradzak-Fleituch, A Dubin, J Owsiński\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A simple method based on rocket and crossed immunoelectrophoresis was adapted for detection of alpha 1 AT-HNE complexes in BALF: Agarose gel with anti-alpha 1 AT combined with intermediate gel containing anti-HNE was used for detection and evaluation of the ratio of complexes with HNE to the free form of alpha 1 AT. BALF samples from 31 patients divided into 5 groups suffering from various respiratory tract diseases were tested. It was found that 3 out of 8 patients with inflammatory pulmonary diseases showed the presence of both forms of alpha 1 AT, 7 out of 13 patients suffering from cancer showed both forms, whereas the complexed form was not detected in any of 8 patients with asthma bronchiale. It is suggested that the presence of alpha 1 AT-HNE complex may reflect, to some extent, the proteolytic status of pulmonary tissue.</p>\",\"PeriodicalId\":7090,\"journal\":{\"name\":\"Acta medica Hungarica\",\"volume\":\"48 1-2\",\"pages\":\"103-9\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1991-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta medica Hungarica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta medica Hungarica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Alpha-1-antitrypsin and alpha-1-antitrypsin-neutrophil elastase complex in bronchoalveolar lavage fluid of patients with pulmonary diseases (pilot study).
A simple method based on rocket and crossed immunoelectrophoresis was adapted for detection of alpha 1 AT-HNE complexes in BALF: Agarose gel with anti-alpha 1 AT combined with intermediate gel containing anti-HNE was used for detection and evaluation of the ratio of complexes with HNE to the free form of alpha 1 AT. BALF samples from 31 patients divided into 5 groups suffering from various respiratory tract diseases were tested. It was found that 3 out of 8 patients with inflammatory pulmonary diseases showed the presence of both forms of alpha 1 AT, 7 out of 13 patients suffering from cancer showed both forms, whereas the complexed form was not detected in any of 8 patients with asthma bronchiale. It is suggested that the presence of alpha 1 AT-HNE complex may reflect, to some extent, the proteolytic status of pulmonary tissue.