{"title":"在实验性GVHR和HVGR大鼠模型中，血清IgG2b和IgM & IgG1 Fc受体在组织肥大细胞上表达增加。","authors":"T Nakatsuji","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Chronic graft-versus-host reaction (GVHR) due to male specific (H-Y) antigen was induced by the injection of syngeneic (DA x Lewis) F1 female cells into (DA x Lewis)F1 male rats. Chronic host-versus-graft reaction (HVGR) based on major histocompatibility complex (MHC) (RT1a) occurred when host Lewis (RT1(1)) rats were transplanted (DA x Lewis)F1 donor cells (RT1a & RT1I). Chronic GVHR and HVGR were activated at fixed periods. The first attacks of the GVHR and HVGR were recognized 50-80 days after cell transplantation, but the most intensive attacks of both responses were observed 120-175 days after cell transplantation. During the most intensive attacks, two rats died from either GVHR or HVGR. Rat immunoglobulin assays measured by radial immunodiffusion (RID) showed that serum IgG2b rose to 10.600-11.500 mg/ml in the rats that had the advanced GVHR or HVGR. The tissue mast cells derived from the loose lymphoid tissues of the medullary and subcapsular sinuses have proliferated in the mesenteric lymph nodes of the rats. IgM and IgG1 binding Fc receptors expressed on the mast cells were demonstrated indirectly using alkaline phosphatase conjugated ant-rat IgM and anti-rat IgG1.</p>","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"64 1","pages":"57-64"},"PeriodicalIF":0.0000,"publicationDate":"1991-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Increased serum IgG2b and IgM & IgG1 Fc receptors expressed on tissue mast cells in experimental GVHR and HVGR rat models.\",\"authors\":\"T Nakatsuji\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Chronic graft-versus-host reaction (GVHR) due to male specific (H-Y) antigen was induced by the injection of syngeneic (DA x Lewis) F1 female cells into (DA x Lewis)F1 male rats. Chronic host-versus-graft reaction (HVGR) based on major histocompatibility complex (MHC) (RT1a) occurred when host Lewis (RT1(1)) rats were transplanted (DA x Lewis)F1 donor cells (RT1a & RT1I). Chronic GVHR and HVGR were activated at fixed periods. The first attacks of the GVHR and HVGR were recognized 50-80 days after cell transplantation, but the most intensive attacks of both responses were observed 120-175 days after cell transplantation. During the most intensive attacks, two rats died from either GVHR or HVGR. Rat immunoglobulin assays measured by radial immunodiffusion (RID) showed that serum IgG2b rose to 10.600-11.500 mg/ml in the rats that had the advanced GVHR or HVGR. The tissue mast cells derived from the loose lymphoid tissues of the medullary and subcapsular sinuses have proliferated in the mesenteric lymph nodes of the rats. IgM and IgG1 binding Fc receptors expressed on the mast cells were demonstrated indirectly using alkaline phosphatase conjugated ant-rat IgM and anti-rat IgG1.</p>\",\"PeriodicalId\":76691,\"journal\":{\"name\":\"The Kitasato archives of experimental medicine\",\"volume\":\"64 1\",\"pages\":\"57-64\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1991-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Kitasato archives of experimental medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Kitasato archives of experimental medicine","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
将同源(DA x Lewis)F1雌性细胞注入(DA x Lewis)F1雄性大鼠体内，诱导雄性特异性(H-Y)抗原引起的慢性移植物抗宿主反应(GVHR)。当宿主Lewis (RT1(1))大鼠移植(DA x Lewis)F1供体细胞(RT1a和RT1I)时，基于主要组织相容性复合体(MHC) (RT1a)的慢性宿主抗移植物反应(HVGR)发生。慢性GVHR和HVGR在固定时间被激活。GVHR和HVGR的首次攻击发生在细胞移植后50 ~ 80天，但两种反应的最强烈攻击发生在细胞移植后120 ~ 175天。在最激烈的攻击期间，两只大鼠死于GVHR或HVGR。径向免疫扩散(RID)法测定大鼠免疫球蛋白，结果显示晚期GVHR或HVGR大鼠血清IgG2b升高至10.600 ~ 11.500 mg/ml。大鼠肠系膜淋巴结内增生的组织肥大细胞来源于髓质和包膜下窦的松散淋巴样组织。利用碱性磷酸酶结合的抗大鼠IgM和抗大鼠IgG1间接证实了肥大细胞上表达的IgM和IgG1结合Fc受体。
Increased serum IgG2b and IgM & IgG1 Fc receptors expressed on tissue mast cells in experimental GVHR and HVGR rat models.
Chronic graft-versus-host reaction (GVHR) due to male specific (H-Y) antigen was induced by the injection of syngeneic (DA x Lewis) F1 female cells into (DA x Lewis)F1 male rats. Chronic host-versus-graft reaction (HVGR) based on major histocompatibility complex (MHC) (RT1a) occurred when host Lewis (RT1(1)) rats were transplanted (DA x Lewis)F1 donor cells (RT1a & RT1I). Chronic GVHR and HVGR were activated at fixed periods. The first attacks of the GVHR and HVGR were recognized 50-80 days after cell transplantation, but the most intensive attacks of both responses were observed 120-175 days after cell transplantation. During the most intensive attacks, two rats died from either GVHR or HVGR. Rat immunoglobulin assays measured by radial immunodiffusion (RID) showed that serum IgG2b rose to 10.600-11.500 mg/ml in the rats that had the advanced GVHR or HVGR. The tissue mast cells derived from the loose lymphoid tissues of the medullary and subcapsular sinuses have proliferated in the mesenteric lymph nodes of the rats. IgM and IgG1 binding Fc receptors expressed on the mast cells were demonstrated indirectly using alkaline phosphatase conjugated ant-rat IgM and anti-rat IgG1.
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