识别阿拉伯莲花叶中的生物碱(Ziziphus spina-christi)。采用UV-VIS光谱学方法

A. M. Andy Suryadi, Mohammad Adam Mustapa, Nur Khofifah Zahrah
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摘要

大枣(Ziziphus spina-christi L.)是一种药用植物,对治疗腹泻、发烧和癌症有很多好处。其中一种起作用的化合物是生物碱。本研究采用紫外-可见分光光度法对红枣叶中的生物碱进行鉴定。用正己烷、乙酸乙酯和甲醇三种极性不同的溶剂浸渍大枣叶。此外,还进行了生物碱类化合物的植物化学筛选。在乙酸乙酯提取物中检出生物碱阳性的红枣叶样品;以正己烷:乙酸乙酯(8:2)为洗脱液进行薄层色谱鉴定,得到4个点,Rf值为0.25;0.4;0.52;和0.62。此外,还进行了生物碱类化合物的植物化学筛选。采用正己烷:乙酸乙酯(8:2)的薄层色谱法鉴定乙酸乙酯提取物中生物碱阳性的红枣,得到4个点,Rf值为0.25;0.4;0.52;和0.62。用紫外-可见分光光度法对4个斑点进行鉴定,得到波长为248;268;268;和255年。根据叶提取物的鉴定结果,每个染色点疑似含有cantin-6- 1生物碱类化合物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
IDENTIFIKASI SENYAWA ALKALOID PADA DAUN BIDARA ARAB (Ziziphus spina-christi L.) MENGGUNAKAN METODE SPEKTROFOTOMETRI UV-VIS
Christ's torn jujube (Ziziphus spina-christi L.) is a medicinal plant that has many benefits for curing or treating diarrhea, fever, and cancer. One of the compounds that play role is an alkaloid. This study was generated to identify the alkaloid compounds in the leaves of the Christ’s jujube torn using the UV-Vis spectrophotometry method. Christ's torn jujube leaves were macerated using 3 types of solvents with different polarity levels i.e., n-hexane, ethyl acetate, and methanol. Furthermore, phytochemical screening of alkaloid compounds was carried out. A Christ jujube leaf sample that was positive for alkaloids was found in the ethyl acetate extract; then identified using thin layer chromatography with eluent n-hexane: ethyl acetate (8:2), yielding 4 points with an Rf value of 0.25; 0.4 ; 0.52 ; and 0.62. Furthermore, phytochemical screening of alkaloid compounds was carried out. Christ' torn jujube which were positive for alkaloids present in the ethyl acetate extract were identified using thin layer chromatography with n-hexane eluent: ethyl acetate (8:2), yielding four spots with an Rf value of 0.25; 0.4 ; 0.52 ; and 0.62. The four spots were identified using UV-Vis spectrophotometry, obtained a wavelength of 248 ; 268 ; 268 ; and 255. Based on the identification results on the leaf extract, each stain was suspected to contain cantin-6-one alkaloid compounds.
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