计算机图像分析在定量显微镜中的应用

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society Pub Date : 1988-11-01 DOI:10.1109/IEMBS.1988.94530

T. Flotte

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引用次数: 1

摘要

通过光学显微镜获得的数字化图像允许对测量参数进行量化。作者一直在研究使用视频密度测定法分析皮肤弹性组织的体积分数，通过不同波长的特殊吸光度比例来定位动脉粥样硬化中b -胡萝卜素的位置，以及荧光团的定量。这些视频密度测量技术在诊断病理学中的应用提供了获得定量信息的可能性，这些信息将在实验室之间重复;然而，这些研究必须在理解硬件和技术的基础上进行分析。

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Application of computerized image analysis in quantitative microscopy

Digitized images obtained through a light microscope permit quantification of measured parameters. The author has been studying the use of video densitometry to analyze volume fractions of elastic tissue in skin, the localization of B-carotene in atherosclerosis by special ratioing of absorbances at different wavelengths, and the quantification of fluorophores. The application of these techniques of video densitometry to diagnostic pathology offers the possibility of obtaining quantitative information which will be reproducible from laboratory to laboratory; however, these studies must be analyzed with an understanding of the hardware and techniques.<>

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Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society

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