种子生化组成指标在油菜细胞质雄性不育杂种优势育种中的应用

S. Vyshnevsky
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摘要

本文介绍了2014-2017年冬季油菜细胞质雄性不育选育源材料的研究成果。资料来源为中国农业科学院饲料与农业研究所的品种、杂交种、单株选育,收集国内外选育样品。以2014- 2016年冬油菜44个基因型为亲本,采用细胞质雄性不育形式进行传粉。育种材料是根据种子产量、生化成分指标、抗寒性、产油性、生长季节长短、病害病原菌的损害程度和对害虫的危害等因素,经过多年加工而成的。为了加快国内竞争性油菜品种和杂交品种的选育进程,以细胞质雄性不育为基础,对F1杂交品种种子的生化参数进行了研究。根据2016年25个组合的芥酸、硫代葡萄糖苷含量指标,分配出7个组合,其中种子生化成分符合要求;不含或微量油芥酸,硫代葡萄糖苷含量不超过25 μmol/g。2017年,在杂种优势表现及在此基础上利用CMS培育高杂种优势杂交种的研究中,利用了19个新杂交种和7个2016年最佳杂交种。在2017年的油品和产量方面,有26个数字突出了15个,其中7个是2016年的。2年试验结果表明:2016年,7个最佳杂交种的总平均产量为7.41 t/ha,比标准高出2.59 t/ha;2017年,这些组合的总平均作物产量为6.58 t/公顷,比标准高出1.8 t/公顷。冬季油菜杂交品种杂种优势表现率平均为46%。选择工作的权宜性决定了创造冬季油菜商业杂交种的来源材料,其中包括早期检测,甚至在第一代杂交种阶段,有希望的细胞质雄性不育的恢复和固定的二零系。本研究为进一步排除已收到的原始材料的生化指标改进工作的选择方案提供了机会。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
USE OF INDICATORS OF BIOCHEMICAL COMPOSITION OF SEEDS FOR BREEDING FOR HETEROSIS WINTER OILSEED RAPE BASED CYTOPLASMIC MALE STERILITY
The paper presents the results of research (2014-2017) on the creation of source material for the selection of hybrids of winter oilseed rape on the basis of cytoplasmic male sterility. The source material was varieties, hybrids, lines of individual selection of the Institute of Feed Research and Agriculture of Podillya NAAS, collection samples of domestic and foreign selection. We used 44 genotypes of winter oilseed rape in 2014-16 as parents for pollination with a form with cytoplasmic male sterility. Material for breeding was taken taking into account many years of processing according to seed productivity, indicators of biochemical composition, winter resistance, oleaginousness, length of growing season, lesion of pathogens of disease and damage to pests. To accelerate the selection process for the creation of competitive domestic varieties and hybrids of winter oilseed rape, studies were conducted on the biochemical parameters of seeds of F1 hybrids obtained on the basis of cytoplasmic male sterility. By indicators of the content of erucic acid, glucosinolates from 25 combinations of 2016, seven combinations are allocated, in which the biochemical composition of the seeds correspond to such requirements; Oil - erucic acid is absent, or its traces are contained, and the content of glucosinolates does not exceed 25 μmol/g. In 2017, in studies on the manifestation of heterosis and the creation of highly heterosis hybrids using CMS on their basis, 19 new hybrids and 7 best ones of 2016 were used. In terms of oil quality and yield in 2017 with 26 numbers have highlighted 15, 7 of them are of 2016. According to the results of a two-year trial, we have the following results: 2016, the total average crop capacity of the 7 best hybrids was 7.41 t/ha, which was 2.59 t/ha higher than the standard; 2017, these combinations showed a total average crop capacity of 6.58 t/ha, which was 1.8 t/ha higher than the standard. The manifestation of heterosis in winter oilseed rape hybrids on average for two years was observed at 46%. The expediency of selection work is determined, to create the source material of commercial hybrids of winter oilseed rape, which includes early detection, even at the stage of first-generation hybrids, promising two-zero lines of restorers and fixers of cytoplasmic male sterility. The study gives the chance in the further exclusion from the selection program of works on improvement of biochemical indicators of the received initial material.
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