从牛肠道中分离出乳酸细菌,以及它们抑制大肠杆菌和Shigella sp细菌的能力

Lulu Fatma Dewi, Sartini Sartini, Rahmiati Rahmiati
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引用次数: 5

摘要

本研究的目的是测定牛肠BAL对大肠杆菌和志贺氏菌的抑制能力。采用圆盘扩散法或Kirby-Bauer法,通过测定纸圆盘周围的抑制区来检测BAL对大肠杆菌和志贺氏菌的抑制能力。采用表格和图片形式对数据进行描述性分析。结果从牛肠中分离得到2株BAL。所有菌株对大肠杆菌和志贺氏菌的抑菌试验均呈阳性,其中编码sp1的菌株对大肠杆菌的抑菌带为7.5 mm,对志贺氏菌的抑菌带为6.8 mm,而编码sp2的菌株对大肠杆菌的抑菌带为8.9 mm,对志贺氏菌的抑菌带为8.0 mm。结果表明,具有sp2编码的乳酸菌对大肠杆菌的抑菌带直径为8.9 mm,对志贺菌的抑菌带直径为8.0 mm。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolasi Bakteri Asam Laktat dari Usus Sapi (Bos taurus) serta Kemampuannya dalam Menghambat Pertumbuhan Bakteri Eschericia coli dan Shigella sp
The purpose of this research was to determine the ability of BAL from cattle intestine (Bos taurus) in inhibiting the growth of Eschericia coli and Shigella sp. Testing of BAL capability in inhibiting growth of Escherichia coli and Shigella sp. using the disc-diffusion method or the Kirby-Bauer method, which was done by measuring the inhibit zone around the paper disc. Data were analyzed descriptively by displaying data in table and picture form. The results obtained 2 isolates of BAL from the cow intestine. All isolates showed positive results when tested for antibacterial against Escherichia coli and Shigella sp. In isolate BAL with code sp1 has inhibition zone against Escherichia coli equal to 7.5 mm and to Shigella sp. of 6.8 mm, whereas in isolate BAL with code sp2 has inhibition zone against Escherichia coli equal to 8.9 mm and to Shigella sp. of 8.0 mm. Based on the results obtained, it can be concluded that isolates of lactic acid bacteria with sp2 code has inhibition zone 8.9 mm in inhibiting Eschericia coli while against bacteria Shigella sp. has a diameter of 8.0 mm.
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