E. Handayani, A. Astuti, Yogawati Printarani Yahwidhi
{"title":"兰科植物Grammatophyllum scriptum lindl的体外培养。在叶面肥料培养基中研究Ambonese香蕉皮","authors":"E. Handayani, A. Astuti, Yogawati Printarani Yahwidhi","doi":"10.56125/tpj.v2i2.31","DOIUrl":null,"url":null,"abstract":"The sustainability of Grammatophyllum scriptum orchids in nature is threatened due to excessive harvesting which results in changes and damage to the orchid's growing habitat. G. scriptum orchid is one of the endemic orchids that has uniqueness and high economic value. In vitro culture propagation of G. scriptum orchids is carried out to multiply and maintain the existence of the orchid. The use of alternative media in vitro culture is carried out to minimize the cost of making the medium and utilize materials available in nature. The utilization of Ambonese banana peel waste that has not been carried out optimally is considered to be one of the choices as an alternative medium in propagation of G. scriptum orchids This study aims to determine the most appropriate combination of foliar fertilizer and banana peel concentration for in vitro multiplication of orchid explants and to examine the effect of the combination of leaf fertilizer and banana peel concentration on the multiplication of orchid explantsVitro. The study will be carried out using a single-factor experimental method with 8 treatments, arranged according to the Complete Randomized Design (RAL). The treatment tried was in vitro culture of G. scriptum orchids on Growmore media and Ambonese banana peels including inner banana peels of 50g/L and 100g/L; outer banana peel 50 g/L and 100 g/L; banana peel combined 50 g / L and 100 g / L. The results showed the addition of Ambon banana peel had an influence on the percentage of life and growth of shoots, leaves and roots of G. scriptum orchid plants Treatment with the addition of a combined banana peel of 50 g / L in leaf fertilizer medium provides the best growth in terms of the percentage of life, plant height growth, leaves and plant roots.","PeriodicalId":136396,"journal":{"name":"Tropical Plantation Journal","volume":"42 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In Vitro Culture of Orchids Grammatophyllum scriptum lindl. from Ambonese banana Peel in Foliar Fertilizer Medium\",\"authors\":\"E. Handayani, A. 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引用次数: 0
摘要
由于过度采收导致兰科植物生长栖息地的变化和破坏,兰科植物在自然界的可持续性受到威胁。金针兰是我国特有的兰花之一,具有独特的经济价值。为了繁殖和维持兰科植物的生存,进行了离体培养繁殖。在体外培养中使用替代培养基是为了尽量减少培养基的制作成本,并利用自然界中可用的材料。利用尚未得到优化利用的Ambonese香蕉皮废弃物,可以作为一种替代培养基,用于兰科植物(G. scriptum兰科植物)的繁殖。本研究旨在确定最适宜的叶面肥和香蕉皮浓度组合,并考察叶面肥和香蕉皮浓度组合对兰科植物外植体体外繁殖的影响。本研究采用单因素试验方法,按完全随机设计(RAL)安排8个处理。试验的处理是在Growmore培养基和Ambonese香蕉皮上分别培养50g/L和100g/L的内香蕉皮;香蕉皮外皮50g /L、100g /L;50 g / L和100 g / L配施香蕉皮对兰科植物的芽、叶、根的生长率和生长率均有影响。结果表明,在叶肥培养基中添加50 g / L配施香蕉皮对兰科植物的生长率、株高生长、叶、根生长均有较好的影响。
In Vitro Culture of Orchids Grammatophyllum scriptum lindl. from Ambonese banana Peel in Foliar Fertilizer Medium
The sustainability of Grammatophyllum scriptum orchids in nature is threatened due to excessive harvesting which results in changes and damage to the orchid's growing habitat. G. scriptum orchid is one of the endemic orchids that has uniqueness and high economic value. In vitro culture propagation of G. scriptum orchids is carried out to multiply and maintain the existence of the orchid. The use of alternative media in vitro culture is carried out to minimize the cost of making the medium and utilize materials available in nature. The utilization of Ambonese banana peel waste that has not been carried out optimally is considered to be one of the choices as an alternative medium in propagation of G. scriptum orchids This study aims to determine the most appropriate combination of foliar fertilizer and banana peel concentration for in vitro multiplication of orchid explants and to examine the effect of the combination of leaf fertilizer and banana peel concentration on the multiplication of orchid explantsVitro. The study will be carried out using a single-factor experimental method with 8 treatments, arranged according to the Complete Randomized Design (RAL). The treatment tried was in vitro culture of G. scriptum orchids on Growmore media and Ambonese banana peels including inner banana peels of 50g/L and 100g/L; outer banana peel 50 g/L and 100 g/L; banana peel combined 50 g / L and 100 g / L. The results showed the addition of Ambon banana peel had an influence on the percentage of life and growth of shoots, leaves and roots of G. scriptum orchid plants Treatment with the addition of a combined banana peel of 50 g / L in leaf fertilizer medium provides the best growth in terms of the percentage of life, plant height growth, leaves and plant roots.