电穿孔对中国仓鼠卵巢细胞影响的电泳研究

2014 16th International Symposium on Antenna Technology and Applied Electromagnetics (ANTEM) Pub Date : 2014-07-13 DOI:10.1109/ANTEM.2014.6887659

E. Salimi, K. Braasch, M. Butler, D. Thomson, G. Bridges

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引用次数: 0

摘要

电穿孔通过在细胞膜上形成允许离子运输的孔来影响细胞的介电特性。介质电泳(DEP)是研究单个生物细胞电穿孔的一种强有力的技术。本文研究了中国仓鼠卵巢(CHO)细胞的频率依赖性反应，并探讨了不同细胞参数对DEP光谱的影响。结果表明，低频DEP(小于200 kHz)用于研究细胞膜电导率，中频DEP (1-30 MHz)用于研究细胞质电导率，高频DEP(大于100 MHz)用于研究细胞质介电常数。

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Dielectrophoresis study of electroporation effects on Chinese hamster ovary cells

Electroporation affects the dielectric properties of cells by creating pores in their membrane which allow transport of ions. Dielectrophoresis (DEP) is a powerful technique to investigate electroporation of single biological cells. Here we study the frequency dependent response of Chinese hamster ovary (CHO) cells and investigate the effect of different cell parameters on the DEP spectra. The results suggest low frequency DEP application (less than 200 kHz) for investigating the cell membrane conductivity, mid frequency DEP application (1-30 MH) for investigating the cell cytoplasm conductivity, and high frequency DEP application (greater than 100 MHz) for investigating cell cytoplasm permittivity.

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2014 16th International Symposium on Antenna Technology and Applied Electromagnetics (ANTEM)

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