一种检测霍乱弧菌溶细胞素的免疫酶法。

Laboratornoe delo Pub Date : 1991-01-01

S N Babitsyn, A O Tsittser, V L Semiotrochev, S G Sedina

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摘要

采用酶免疫分析法检测霍乱弧菌溶细胞素。采用高碘酸盐技术，将免疫球蛋白与辣根过氧化物酶结合纯化细胞溶酶。方法灵敏度为纯化的溶细胞素2 ng/ml。40株霍乱弧菌上清溶血活性测定结果与酶免疫分析结果高度相关。

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[An immunoenzyme method of detecting Vibrio cholerae cytolysin].

Enzyme immunoassay was used in detection of V. cholerae cytolysin. Conjugate of immunoglobulins to purified cytolysin with horseradish peroxidase was used, obtained by the periodate technique. The method sensitivity is 2 ng/ml of purified cytolysin. The results of hemolytic activity measurements in supernatants of 40 V. cholerae strains and enzyme immunoassay findings were in high correlation.

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Laboratornoe delo

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