石英晶体微天平检测流感病毒亚型

2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS) Pub Date : 2014-11-01 DOI:10.1109/MHS.2014.7006130

Shuichiro Kagiyama, T. Masuda, K. Kaihatsu, N. Kato, F. Arai

{"title":"石英晶体微天平检测流感病毒亚型","authors":"Shuichiro Kagiyama, T. Masuda, K. Kaihatsu, N. Kato, F. Arai","doi":"10.1109/MHS.2014.7006130","DOIUrl":null,"url":null,"abstract":"We fabricated a microfluidic chip to detect purified influenza virus RNA using Quartz Crystal Microbalance (QCM). The channel made of PDMS (poly dimethylsiloxane) was combined with QCM directly. The targeted virus genome introduced to the channel was captured by Peptide Nucleic Acid (PNA) immobilized on the electrode of QCM. After this, we introduced the gold colloid to be captured by influenza virus RNA to increase weight of virus RNA. By this, we could detect influenza virus RNA (106 pfu/mL).","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Detection of influenza virus subtype using quartz crystal microbalance\",\"authors\":\"Shuichiro Kagiyama, T. Masuda, K. Kaihatsu, N. Kato, F. Arai\",\"doi\":\"10.1109/MHS.2014.7006130\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We fabricated a microfluidic chip to detect purified influenza virus RNA using Quartz Crystal Microbalance (QCM). The channel made of PDMS (poly dimethylsiloxane) was combined with QCM directly. The targeted virus genome introduced to the channel was captured by Peptide Nucleic Acid (PNA) immobilized on the electrode of QCM. After this, we introduced the gold colloid to be captured by influenza virus RNA to increase weight of virus RNA. By this, we could detect influenza virus RNA (106 pfu/mL).\",\"PeriodicalId\":181514,\"journal\":{\"name\":\"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)\",\"volume\":\"1 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2014-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/MHS.2014.7006130\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/MHS.2014.7006130","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 1

摘要

利用石英晶体微天平(QCM)制备了一种检测纯化流感病毒RNA的微流控芯片。将PDMS(聚二甲基硅氧烷)与QCM直接结合。引入通道的目标病毒基因组被固定在QCM电极上的肽核酸(PNA)捕获。在此之后，我们引入了被流感病毒RNA捕获的金胶体，以增加病毒RNA的重量。通过这种方法，我们可以检测到流感病毒RNA (106 pfu/mL)。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Detection of influenza virus subtype using quartz crystal microbalance

We fabricated a microfluidic chip to detect purified influenza virus RNA using Quartz Crystal Microbalance (QCM). The channel made of PDMS (poly dimethylsiloxane) was combined with QCM directly. The targeted virus genome introduced to the channel was captured by Peptide Nucleic Acid (PNA) immobilized on the electrode of QCM. After this, we introduced the gold colloid to be captured by influenza virus RNA to increase weight of virus RNA. By this, we could detect influenza virus RNA (106 pfu/mL).

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)

自引率

0.00%

发文量