{"title":"青春期后小鼠早期腔前卵泡在简化培养系统中的体外发育","authors":"H. Kada","doi":"10.3192/JSIRIB2003.20.1_1","DOIUrl":null,"url":null,"abstract":"The developmental competence in vitro of immature oocytes from mouse early preantral follicles in a simplified culture was investigated. The follicles ware obtained from 4-10 weeks and 14 days old mice, respectively. On day 12 of culture, most of cultured follicles ware survived (100% vs 97%) in this culture system. The granulosa cell outgrowth was observed in all of surviving follicles. The oocytes grown in vitro ware leaded to GVBD (84% vs 96%), and 44% of them reached Metaphase II, respectively. This results show that this culture system is valid in not only preantaral follicles from prepuberal mouse but also that of postpuberal periods.","PeriodicalId":126001,"journal":{"name":"Reproductive Immunology and Biology","volume":"71 5 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In-vitro development of early preantral ovarian follicles from postpuberal mice in a simplified culture system\",\"authors\":\"H. Kada\",\"doi\":\"10.3192/JSIRIB2003.20.1_1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The developmental competence in vitro of immature oocytes from mouse early preantral follicles in a simplified culture was investigated. The follicles ware obtained from 4-10 weeks and 14 days old mice, respectively. On day 12 of culture, most of cultured follicles ware survived (100% vs 97%) in this culture system. The granulosa cell outgrowth was observed in all of surviving follicles. The oocytes grown in vitro ware leaded to GVBD (84% vs 96%), and 44% of them reached Metaphase II, respectively. This results show that this culture system is valid in not only preantaral follicles from prepuberal mouse but also that of postpuberal periods.\",\"PeriodicalId\":126001,\"journal\":{\"name\":\"Reproductive Immunology and Biology\",\"volume\":\"71 5 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1900-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Reproductive Immunology and Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3192/JSIRIB2003.20.1_1\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reproductive Immunology and Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3192/JSIRIB2003.20.1_1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
研究了小鼠早期腔前卵泡未成熟卵母细胞体外简化培养的发育能力。卵泡分别取自4-10周龄和14日龄小鼠。培养第12天，大部分卵泡成活(100% vs 97%)。在所有存活的卵泡中均可见颗粒细胞的生长。体外培养的卵母细胞导致GVBD (84% vs 96%)，分别有44%达到中期II。结果表明，该培养体系不仅适用于青春期前小鼠的卵泡，而且适用于青春期后小鼠的卵泡。
In-vitro development of early preantral ovarian follicles from postpuberal mice in a simplified culture system
The developmental competence in vitro of immature oocytes from mouse early preantral follicles in a simplified culture was investigated. The follicles ware obtained from 4-10 weeks and 14 days old mice, respectively. On day 12 of culture, most of cultured follicles ware survived (100% vs 97%) in this culture system. The granulosa cell outgrowth was observed in all of surviving follicles. The oocytes grown in vitro ware leaded to GVBD (84% vs 96%), and 44% of them reached Metaphase II, respectively. This results show that this culture system is valid in not only preantaral follicles from prepuberal mouse but also that of postpuberal periods.
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