{"title":"磁性表面印迹水凝胶纳米颗粒特异性和可逆稳定的蛋白质","authors":"Yibin Liu, Junqiu Zhai, Jiantong Dong, Meiping Zhao","doi":"10.1515/molim-2015-0006","DOIUrl":null,"url":null,"abstract":"Abstract In this work, we demonstrate the unique capability of surface imprinted hydrogels over magnetic nanoparticles (Fe3O4@MIP-NPs) for protection of enzyme proteins from denaturation induced by heat, UV irradiation, ultrasonication and addition of urea. Moreover, the Fe3O4@MIP-NPs successfully enabled rapid renaturation of heat-denatured proteins under mild conditions. These interesting properties of MIP nanoparticles offer useful tools for protection and renaturation of proteins in various applications.","PeriodicalId":155658,"journal":{"name":"Molecular Imprinting","volume":"15 12 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2015-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":"{\"title\":\"Magnetic surface imprinted hydrogel nanoparticles for specific and reversible stabilization of proteins\",\"authors\":\"Yibin Liu, Junqiu Zhai, Jiantong Dong, Meiping Zhao\",\"doi\":\"10.1515/molim-2015-0006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract In this work, we demonstrate the unique capability of surface imprinted hydrogels over magnetic nanoparticles (Fe3O4@MIP-NPs) for protection of enzyme proteins from denaturation induced by heat, UV irradiation, ultrasonication and addition of urea. Moreover, the Fe3O4@MIP-NPs successfully enabled rapid renaturation of heat-denatured proteins under mild conditions. These interesting properties of MIP nanoparticles offer useful tools for protection and renaturation of proteins in various applications.\",\"PeriodicalId\":155658,\"journal\":{\"name\":\"Molecular Imprinting\",\"volume\":\"15 12 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular Imprinting\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1515/molim-2015-0006\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Imprinting","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/molim-2015-0006","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Magnetic surface imprinted hydrogel nanoparticles for specific and reversible stabilization of proteins
Abstract In this work, we demonstrate the unique capability of surface imprinted hydrogels over magnetic nanoparticles (Fe3O4@MIP-NPs) for protection of enzyme proteins from denaturation induced by heat, UV irradiation, ultrasonication and addition of urea. Moreover, the Fe3O4@MIP-NPs successfully enabled rapid renaturation of heat-denatured proteins under mild conditions. These interesting properties of MIP nanoparticles offer useful tools for protection and renaturation of proteins in various applications.
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