{"title":"尸体器官的DNA:两种提取方法的比较。","authors":"G Kinzinger, J Holtz","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Genomic DNA was isolated from tissue specimen by two different methods. The rapid extraction according to Higuchi provided increased product yield of DNA in a better quality than the extraction with phenol/chloroform. Comparing the organ tissues according to quantity and quality of DNA it was found that best yield was recovered from brain tissue followed by lung and liver. DNA stability is directly influenced by postmortal age and age of the bodies.</p>","PeriodicalId":75580,"journal":{"name":"Beitrage zur gerichtlichen Medizin","volume":"50 ","pages":"283-7"},"PeriodicalIF":0.0000,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[DNA of cadaver organs: comparison of two methods of extraction].\",\"authors\":\"G Kinzinger, J Holtz\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Genomic DNA was isolated from tissue specimen by two different methods. The rapid extraction according to Higuchi provided increased product yield of DNA in a better quality than the extraction with phenol/chloroform. Comparing the organ tissues according to quantity and quality of DNA it was found that best yield was recovered from brain tissue followed by lung and liver. DNA stability is directly influenced by postmortal age and age of the bodies.</p>\",\"PeriodicalId\":75580,\"journal\":{\"name\":\"Beitrage zur gerichtlichen Medizin\",\"volume\":\"50 \",\"pages\":\"283-7\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1992-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Beitrage zur gerichtlichen Medizin\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Beitrage zur gerichtlichen Medizin","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[DNA of cadaver organs: comparison of two methods of extraction].
Genomic DNA was isolated from tissue specimen by two different methods. The rapid extraction according to Higuchi provided increased product yield of DNA in a better quality than the extraction with phenol/chloroform. Comparing the organ tissues according to quantity and quality of DNA it was found that best yield was recovered from brain tissue followed by lung and liver. DNA stability is directly influenced by postmortal age and age of the bodies.
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