P. Mulder, G. Molema, S. Koster, H.J. van der Linden, E. Verpoorte
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Behaviour of Human Umbilical Vein Endothelial Cells (HUVEC) Cultivated in Microfluidic channels
Our long-term goal is to develop advanced tools for cell studies and analysis based on microfluidic systems. In this paper, we report on endothelial cell cultivation in microchannels and 96-well tissue plates, and compare cell phenotype and cellular status in the two environments This was done under both pro-inflammatory conditions (cell stimulation in the presence of the cytokine, Tumour Necrosis Factor, TNFalpha) and normal (medium only) conditions. In addition, we considered the behaviour of cells in a pro-inflammatory environment in the presence of an anti-inflammatory drug
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