{"title":"从沙特阿拉伯利雅得医院收集的铜绿假单胞菌临床分离物无蛋白细胞培养物中外毒素 A 的发生率和水平。","authors":"R M al-Ssum","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Mice lethality bioassay revealed that out of 102 proteolytic cell-free cultures of Pseudomonas aeruginosa only 17 (15%) were lethal with values of 19-48 LD50/ml. Antitoxin A antiserum neutralized the lethality providing evidence that the main lethal compound in the cell free cultures was exotoxin A. The neutralization also ruled out the lethal contribution of protease, elastase and phospholipase c. The presence of exotoxin A in a proteolytic environment may indicate less efficient proteolytic action at the stage of toxin production.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"15 4","pages":"355-9"},"PeriodicalIF":0.0000,"publicationDate":"1992-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Incidence and level of exotoxin A in proteolytic cell-free cultures of clinical isolates of Pseudomonas aeruginosa collected from hospitals in Riyadh, Saudi Arabia.\",\"authors\":\"R M al-Ssum\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Mice lethality bioassay revealed that out of 102 proteolytic cell-free cultures of Pseudomonas aeruginosa only 17 (15%) were lethal with values of 19-48 LD50/ml. Antitoxin A antiserum neutralized the lethality providing evidence that the main lethal compound in the cell free cultures was exotoxin A. The neutralization also ruled out the lethal contribution of protease, elastase and phospholipase c. The presence of exotoxin A in a proteolytic environment may indicate less efficient proteolytic action at the stage of toxin production.</p>\",\"PeriodicalId\":77264,\"journal\":{\"name\":\"Microbiologica\",\"volume\":\"15 4\",\"pages\":\"355-9\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1992-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbiologica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiologica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
小鼠致死率生物测定显示，在 102 个铜绿假单胞菌蛋白水解无细胞培养物中，只有 17 个（15%）具有致死率，半数致死剂量为 19-48 LD50/ml。抗毒素 A 抗血清中和了致死率，证明无细胞培养物中的主要致死化合物是外毒素 A。
Incidence and level of exotoxin A in proteolytic cell-free cultures of clinical isolates of Pseudomonas aeruginosa collected from hospitals in Riyadh, Saudi Arabia.
Mice lethality bioassay revealed that out of 102 proteolytic cell-free cultures of Pseudomonas aeruginosa only 17 (15%) were lethal with values of 19-48 LD50/ml. Antitoxin A antiserum neutralized the lethality providing evidence that the main lethal compound in the cell free cultures was exotoxin A. The neutralization also ruled out the lethal contribution of protease, elastase and phospholipase c. The presence of exotoxin A in a proteolytic environment may indicate less efficient proteolytic action at the stage of toxin production.
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