C. Robinson Edward Raja, Ravi Pandeeswari̇, U. Ramesh
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引用次数: 1
摘要
地下水氟化物污染是国内外主要的毒理学环境问题之一。从内森塔鲁克抽取水样，筛选耐氟细菌。最初，从50 mM NaF补充LB琼脂板上选择24个抗氟菌落。所有分离株血琼脂板均显示β溶血，证实为病原菌。用PCR方法鉴定了毒力因子(algD、plcH、toxA、gyrB、rhlC、lasB)和生物膜形成基因(ppyR、pelA、pslA)。通过基因特异性PCR，成功地从这些分离物中扩增出氟离子转运体crcB。假单胞菌属和铜绿假单胞菌属均属铜绿假单胞菌属。此外，根据高氟耐药筛选出3株高氟耐药菌株，经16S rRNA测序和NCBI blast分析确认为铜绿假单胞菌。
Characterization of high fluoride resistant Pseudomonas aeruginosa species isolated from water samples
Groundwater fluoride contamination is one of the dominant toxicological environmental problems in India and overseas. Water samples were taken from Natham taluk and screened the fluoride resistant bacteria. Initially, Twenty- four fluoride resistant colonies were selected from 50 mM NaF supplemented LB agar plates. All isolates showed β-haemolysis on blood agar plates, confirmed as pathogens. Virulence factors (algD, plcH, toxA, gyrB, rhlC, lasB) and biofilm-forming genes (ppyR, pelA, pslA) were identified in these isolates by PCR analysis. Fluoride ion transporter ‘crcB’ was successfully amplified from these isolates by gene-specific PCR. Genus Pseudomonas and P. aeruginosa species-specific PCR analysis identified that all strains belonging to P. aeruginosa species. Besides, three high fluoride resistance strains were selected based on high fluoride resistance and confirmed as P. aeruginosa species by 16S rRNA sequencing and NCBI blast analysis.
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