Cerberus头诱导剂是一种多价细胞外抑制剂

E. Agius, Stefano Piccolo, E. D. Robertis
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引用次数: 3

摘要

头部诱导剂Cerberus是一种多价细胞外抑制剂,Cerberus编码一种分泌蛋白,当在爪蟾胚胎中腹侧过度表达时,该蛋白会诱导没有躯干的头分化(Bouwmeester et al., 1996)。我们最近发现,Cerberus可以在细胞外空间结合BMP-4 (Bone Morphogenetic Protein-4)、Xnr-1 (Xenopus Nodal-related 1)和Xwnt-8 (Piccolo等,1999)。我们在这里提出的研究表明,Cerberus没有受体,也没有专门的转导途径,而是作为细胞外抑制剂。我们的研究结果表明,Cerberus在头部诱导中的作用可以通过抑制上游节点相关受体和BMP-4受体的活性来解释。此外,利用显性阴性受体突变体阻断xr -1和BMP-4转导途径,我们发现这种双重抑制足以诱导腹侧中胚层外植体的头部诱导。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
L'inducteur céphalique Cerberus est un inhibiteur multivalent extracellulaire
The head inducer Cerberus is a multivalent extracellular inhibitor Cerberus encodes for a secreted protein which when overexpressed ventrally in a Xenopus embryo induces head differentiation without trunk (Bouwmeester et al., 1996). We have recently shown that Cerberus can bind BMP-4 (Bone Morphogenetic Protein-4), Xnr-1 (Xenopus Nodal-related 1) and Xwnt-8 in the extracellular space (Piccolo et al., 1999). We present here studies showing that Cerberus does not have a receptor nor a dedicated transduction pathway but rather acts as an extracellular inhibitor. Our results suggest that the action of Cerberus in head induction can be explained by an inhibitory activity upstream of the Nodal-related and BMP-4 receptors. In addition, using dominant negative receptor mutants which block both the Xnr-1 and BMP-4 transduction pathways, we show that this double inhibition is sufficient for head induction in ventral mesoderm explants.
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