中性缓冲福尔马林(NBF 10%)和Helly溶液固定小鼠肝组织的显微图像

Melly Agustin
{"title":"中性缓冲福尔马林(NBF 10%)和Helly溶液固定小鼠肝组织的显微图像","authors":"Melly Agustin","doi":"10.31983/jlm.v3i2.8053","DOIUrl":null,"url":null,"abstract":"The fixation solution that is widely used in anatomical pathology laboratories is NBF 10%, the excess of NBF 10% because the pH is close to neutral, can be stored in large quantities and a long time. Helly's fixation solution is a good fixation solution for the cytoplasm, and only requires 2-3 hours of fixation. Knowing the microscopic picture of the preparation of hepar mencit tissue (Mus musculus) fixated with NBF 10% and Helly solution. This research is an experimental study with a descriptive analysis approach. picture of hepar mencit tissue preparation (Mus musculus) fixation with NBF 10% obtained as much as 100% good preparation. While the fixated with Helly solution obtained as much as 66% good preparation. Conclusion: Microscopic picture of hepar mencit tissue preparation (Mus musculus) fixated with NBF is 10% better than Helly solution.","PeriodicalId":232833,"journal":{"name":"Jaringan Laboratorium Medis","volume":"10 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2021-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Microscopic Profile of Mice Liver Tissue (Mus musculus) Fixed with Neutral Buffered Formalin (NBF 10%) and Helly Solution\",\"authors\":\"Melly Agustin\",\"doi\":\"10.31983/jlm.v3i2.8053\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The fixation solution that is widely used in anatomical pathology laboratories is NBF 10%, the excess of NBF 10% because the pH is close to neutral, can be stored in large quantities and a long time. Helly's fixation solution is a good fixation solution for the cytoplasm, and only requires 2-3 hours of fixation. Knowing the microscopic picture of the preparation of hepar mencit tissue (Mus musculus) fixated with NBF 10% and Helly solution. This research is an experimental study with a descriptive analysis approach. picture of hepar mencit tissue preparation (Mus musculus) fixation with NBF 10% obtained as much as 100% good preparation. While the fixated with Helly solution obtained as much as 66% good preparation. Conclusion: Microscopic picture of hepar mencit tissue preparation (Mus musculus) fixated with NBF is 10% better than Helly solution.\",\"PeriodicalId\":232833,\"journal\":{\"name\":\"Jaringan Laboratorium Medis\",\"volume\":\"10 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-11-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Jaringan Laboratorium Medis\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.31983/jlm.v3i2.8053\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jaringan Laboratorium Medis","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.31983/jlm.v3i2.8053","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

解剖病理实验室广泛使用的固定液为NBF 10%,超过NBF 10%由于pH值接近中性,可以大量、长时间保存。Helly’s固定液是一种很好的细胞质固定液,只需要固定2-3小时。了解10% NBF和Helly溶液固定肝包膜组织制备的显微图像。本研究采用描述性分析方法进行实验研究。肝包膜组织准备物(小家鼠)固定,NBF 10%,获得100%的良好准备。而用Helly溶液固定得到的准备率高达66%。结论:NBF固定肝包膜组织(小家鼠)的显微成像效果比Helly溶液好10%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microscopic Profile of Mice Liver Tissue (Mus musculus) Fixed with Neutral Buffered Formalin (NBF 10%) and Helly Solution
The fixation solution that is widely used in anatomical pathology laboratories is NBF 10%, the excess of NBF 10% because the pH is close to neutral, can be stored in large quantities and a long time. Helly's fixation solution is a good fixation solution for the cytoplasm, and only requires 2-3 hours of fixation. Knowing the microscopic picture of the preparation of hepar mencit tissue (Mus musculus) fixated with NBF 10% and Helly solution. This research is an experimental study with a descriptive analysis approach. picture of hepar mencit tissue preparation (Mus musculus) fixation with NBF 10% obtained as much as 100% good preparation. While the fixated with Helly solution obtained as much as 66% good preparation. Conclusion: Microscopic picture of hepar mencit tissue preparation (Mus musculus) fixated with NBF is 10% better than Helly solution.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信