{"title":"纳米结构与碳质生物传感器-酚类化合物检测的比较研究","authors":"C. Apetrei, J. Saja, M. L. Rodriguez-Mendez","doi":"10.5220/0003716701040109","DOIUrl":null,"url":null,"abstract":"The biosensing properties of tyrosinase biosensors were investigated for two different immobilization matrixes: carbon paste and Langmuir-Blodgett thin film. In both cases the electron mediator was the lutetium (III) bisphthalocyaninate. The electrochemical responses of biosensors towards phenol and catechol were analyzed and compared. The tyrosinase maintains its bioactivity well within the immobilization matrices. A clearly defined reduction current proportional to the phenolic compounds concentration was observed in cyclic voltammetry, which attributed to the reduction of enzymatically produced quinone at the electrode surface. It was demonstrated that the biosensor based on LangmuirBlodgett thin film shows the best performances in terms of kinetics and detection limit for the phenolic","PeriodicalId":357085,"journal":{"name":"International Conference on Biomedical Electronics and Devices","volume":"39 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2016-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Nanostructured vs. Carbonaceous Biosensors - Comparative Studies for Detection of Phenolic Compounds\",\"authors\":\"C. Apetrei, J. Saja, M. L. Rodriguez-Mendez\",\"doi\":\"10.5220/0003716701040109\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The biosensing properties of tyrosinase biosensors were investigated for two different immobilization matrixes: carbon paste and Langmuir-Blodgett thin film. In both cases the electron mediator was the lutetium (III) bisphthalocyaninate. The electrochemical responses of biosensors towards phenol and catechol were analyzed and compared. The tyrosinase maintains its bioactivity well within the immobilization matrices. A clearly defined reduction current proportional to the phenolic compounds concentration was observed in cyclic voltammetry, which attributed to the reduction of enzymatically produced quinone at the electrode surface. It was demonstrated that the biosensor based on LangmuirBlodgett thin film shows the best performances in terms of kinetics and detection limit for the phenolic\",\"PeriodicalId\":357085,\"journal\":{\"name\":\"International Conference on Biomedical Electronics and Devices\",\"volume\":\"39 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-11-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Conference on Biomedical Electronics and Devices\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5220/0003716701040109\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Conference on Biomedical Electronics and Devices","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5220/0003716701040109","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Nanostructured vs. Carbonaceous Biosensors - Comparative Studies for Detection of Phenolic Compounds
The biosensing properties of tyrosinase biosensors were investigated for two different immobilization matrixes: carbon paste and Langmuir-Blodgett thin film. In both cases the electron mediator was the lutetium (III) bisphthalocyaninate. The electrochemical responses of biosensors towards phenol and catechol were analyzed and compared. The tyrosinase maintains its bioactivity well within the immobilization matrices. A clearly defined reduction current proportional to the phenolic compounds concentration was observed in cyclic voltammetry, which attributed to the reduction of enzymatically produced quinone at the electrode surface. It was demonstrated that the biosensor based on LangmuirBlodgett thin film shows the best performances in terms of kinetics and detection limit for the phenolic