更多关于储存时间和温度对尿酶的影响:一项为期1年的研究。

Enzyme Pub Date : 1992-01-01 DOI:10.1159/000468796
E Matteucci, L Pellegrini, C Uncini-Manganelli, M Cecere, M Saviozzi, O Giampietro
{"title":"更多关于储存时间和温度对尿酶的影响:一项为期1年的研究。","authors":"E Matteucci,&nbsp;L Pellegrini,&nbsp;C Uncini-Manganelli,&nbsp;M Cecere,&nbsp;M Saviozzi,&nbsp;O Giampietro","doi":"10.1159/000468796","DOIUrl":null,"url":null,"abstract":"<p><p>Results of our conclusive study on urinary enzyme stability during sample storage are reported. We measured alanine aminopeptidase (AAP) and N-acetyl-beta-D-glucosaminidase (NAG) in morning urines from 9 healthy normal subjects immediately after collection and throughout a 1-year storage at -70 and -20 degrees C. AAP proved to be quite stable at -70 degrees C (99.2% of the basal value at the end of the year). NAG is partially preserved (84.1% of the basal value) at -70 degrees C, but significantly decreased (50.4%) at -20 degrees C.</p>","PeriodicalId":11933,"journal":{"name":"Enzyme","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000468796","citationCount":"8","resultStr":"{\"title\":\"More on effects of storage time and temperature on urinary enzymes: a 1-year study.\",\"authors\":\"E Matteucci,&nbsp;L Pellegrini,&nbsp;C Uncini-Manganelli,&nbsp;M Cecere,&nbsp;M Saviozzi,&nbsp;O Giampietro\",\"doi\":\"10.1159/000468796\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Results of our conclusive study on urinary enzyme stability during sample storage are reported. We measured alanine aminopeptidase (AAP) and N-acetyl-beta-D-glucosaminidase (NAG) in morning urines from 9 healthy normal subjects immediately after collection and throughout a 1-year storage at -70 and -20 degrees C. AAP proved to be quite stable at -70 degrees C (99.2% of the basal value at the end of the year). NAG is partially preserved (84.1% of the basal value) at -70 degrees C, but significantly decreased (50.4%) at -20 degrees C.</p>\",\"PeriodicalId\":11933,\"journal\":{\"name\":\"Enzyme\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1992-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1159/000468796\",\"citationCount\":\"8\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Enzyme\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1159/000468796\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Enzyme","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1159/000468796","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 8

摘要

我们结论性的研究结果尿酶稳定性在样品储存期间报告。在-70℃和-20℃条件下,我们立即测量了9名健康正常受试者晨尿中的丙氨酸氨基肽酶(AAP)和n -乙酰- β - d -氨基葡萄糖苷酶(NAG),并在-70℃条件下进行了1年的储存。结果表明,AAP在-70℃条件下相当稳定(年底基础值的99.2%)。NAG在-70℃时部分保存(基本值的84.1%),但在-20℃时显著降低(50.4%)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
More on effects of storage time and temperature on urinary enzymes: a 1-year study.

Results of our conclusive study on urinary enzyme stability during sample storage are reported. We measured alanine aminopeptidase (AAP) and N-acetyl-beta-D-glucosaminidase (NAG) in morning urines from 9 healthy normal subjects immediately after collection and throughout a 1-year storage at -70 and -20 degrees C. AAP proved to be quite stable at -70 degrees C (99.2% of the basal value at the end of the year). NAG is partially preserved (84.1% of the basal value) at -70 degrees C, but significantly decreased (50.4%) at -20 degrees C.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信