尼日利亚索科托州鸡传染性支气管炎病毒血清学调查

H. U. Mungadi, Salisu M. Garba, Z. Shehu, U. Ahmad, A. Jimoh, E. Ibitoye, A. Shuaibu, Rabi’at Zubair, M. Salman
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摘要

在本研究中,采集了400只鸡的血液样本,并使用基于IgG的间接酶联免疫吸附试验(ELISA)采集血清进行血清学分析。本研究旨在确定尼日利亚索科托州鸡传染性支气管炎病毒抗体的流行情况。样本是从索科托州四个农业区的选定地区收集的。总血清患病率为346例(86.50%)。本地鸡和外来鸡传染性支气管炎病毒抗体血清阳性率分别为88.72%和84.39%,两组间χ 2差异无统计学意义,χ 2值为1.603,P值为0.205(>0.05%)。雏鸡和成鸡血清患病率分别为89.50%和82.87%,卡方呈正相关,chi值为3.179,P值为0.038(0.05)。阳性阳性率分别为86.88%和86.17%,雄鸡略高于雌鸡,χ 2 χ 2分析差异无统计学意义(χ 2 = 0.043, P = 0.836,均>0.05%)。这项研究提供了索科托州IBV血清流行情况的最新情况。结论是,在该州抽样的鸡中,IBV的血清流行率很高。建议在索科托所属的整个地区(尼日利亚西北部)或整个国家对病毒进行全面调查,以便更清楚地了解乙型肝炎,兽医可能认为接种疫苗是预防该病的一种手段,并使用当地菌株生产疫苗
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Serological Survey of Infectious Bronchitis Virus in Chickens in Sokoto State Nigeria
In the present study, blood samples were collected from 400 chickens and sera harvested for serology using an IgG based indirect enzyme linked immunosorbent assay (ELISA). This research aimed at determining the prevalence of antibodies to infectious bronchitis virus in chickens in sokoto state Nigeria. The samples were collected from selected areas in each of the four Agricultural zones of Sokoto State. The overall sero-prevalence obtained was 346 (86.50%). The sero-prevalence of infectious bronchitis viral antibodies in indigenous and exotic chickens was 88.72% and 84.39% respectively, chi square showed no significant relationship between the two groups, the chi value was 1.603 and P value was 0.205 (>0.05%). In young and adult chickens sampled, the sero-prevalence was 89.50% and 82.87% respectively, chi square showed significant relationship between the two age groups with chi value 3.179 and P value 0.038 (<0.05), binary logistic regression for the two age groups showed no significant association with P= 0.09 (>0.05). The result in male and female chickens tested was 86.88% and 86.17% positives respectively showing slightly higher positives in males than in females with no significant statistical relationship using chi square, chi value was 0,043 and P value 0.836 (>0.05%). This study has provided an update on sero-prevalence of IBV in Sokoto State. It was concluded that there was high sero-prevalence of IBV in the chickens sampled in the State. General survey of the virus in the entire region where Sokoto belongs (Northwestern Nigeria) or the country as a whole is recommended, so as to have a clearer picture of IB and Veterinarians probably consider vaccination as a mean of prevention of the disease, and vaccine be produced using local strains
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