EARLY DIAGNOSIS OF MYOCARDIAL INFARCTION

Myocardial infarction (MI) is caused when the blood flow to a segment of the myocardium is disrupted .It can be diagnosed earlier by the excess release of a protein named troponin in the blood. Tests for cardiac biomarker (Troponin I) can also be used to help determine a person's risk of having these conditions or to help monitor and manage someone with suspected MI. The main aim of this thesis is to develop a point-of-care device for rapid detection of Myocardial Infarction by means of protein named troponin I (cTnI).MI is characterized by damage to the cardiac myocytes, which causes the troponins to enter the circulation, where they can be detected using biosensor (Surface Plasmon Resonance). SPR technique measures the refractive index of very thin layers of material absorbed on metal. Antibodies have been immobilized on the surface of a thin metal film, deposited on the reflecting surface of the glass prism, When interactions between antigen and immobilized antibodies occur change in the refractive index occurs, Variation in light intensity reflecting from the back of the film will be detected. This led to the development of bedside troponin I testing methods Index Terms – Myocardial infarction,Biomarker, Biosensor, SPR, Troponin I, Cardiac myocytes.