Isolation, Identification, and Antibiotic Sensitivity Pattern of Salmonella spp from Locally Isolated Egg Samples

Salmonella has been found to be the major foodborne disease in the world with a serious public health problem. The presence of any serotype of Salmonella in food under that food unfit for human consumption. Poultry eggs are considered as major sources for these pathogenic microorganisms. The current study was carried out to isolate and identify the Salmonella spp from egg sample collected from different retailer markets and farm of Savar area, Bangladesh. Antibiotic susceptibility test was also done to determine the resistance pattern. A total of 50 poultry eggs were examined. Salmonella spp were isolated by culturing on the selective plate and characterized by biochemical tests. In the present study, the average prevalence of Salmonella was found to be 100% from the outer surface of eggs and 20% from eggs yolk. All identified isolates were tested for antibiotic susceptibility to six commonly used antimicrobials by the disk diffusion technique. The highest percentage of resistance (60%) was found to Chloramphenicol, Ampicillin, Gentamicin, and Tetracycline. Salmonella isolated from egg surface were found more antibiotic resistant than that of egg yolk. The present study suggests that poultry eggs are a potential reservoir of antibiotic-resistant Salmonella.