[测量工作区空气中茜素浓度的光度法和色谱法]。

E G Ivaniuk, Iu A Kolievskaia, N E Pisareva, S V Mikhaĭlik, I D Perets
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引用次数: 0

摘要

采用两种方法评价乙酰赖氨酸水平。分光光度法与对硝基苯二钠反应,测定产物在510 nm处的光密度。二是薄层色谱法,以正丁醇、甲酸和水的混合物为洗脱液,以0.2%丁醇溶液和3ml冰醋酸(60-70℃)为显像剂。两种方法都适用于在半MAC和外加剂存在的情况下,对工作区域空气中乙酰赖氨酸水平的评价。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Photometric and chromatographic methods of measuring the concentration of acelysin in the air of a work area].

Two methods were applied to evaluation of acelysine level. Spectrophotometric one was based on reaction with p-nitrophenyldiasonium and evaluation of the product optic density at 510 nm. The second was chromatography in thin layer, where a mixture of n-butanol, formic acid and water was used as an eluent and 0.2% ninhydrin solution in butanol and 3 ml of ice acetic acid (60-70 degrees C) served as a developer. Both methods are supposed to evaluate acelysine level in the air of working area, when a half of MAC and admixtures are present.

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