L R Lebedev, I S Andreeva, G N Afinogenova, Iu P Zernov, G D Serov, V E Chizhikov, N M Pustoshilova
{"title":"限制性内切酶:新的生产菌株的检测和酶制剂的分离。","authors":"L R Lebedev, I S Andreeva, G N Afinogenova, Iu P Zernov, G D Serov, V E Chizhikov, N M Pustoshilova","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The method for analysis of microorganisms for the presence of the modification-restriction systems has been developed. The method has permitted to detect more than 10 new producing strains of restrictases including microorganisms of Rhizobium genus. Some of them are promising for practical use. It has been shown that using selection of clones the strain productivity can be increased. The purification process for the majority of restrictases has been proposed. Some physical and catalytic properties of new enzymes have been studied.</p>","PeriodicalId":18920,"journal":{"name":"Nauchnye doklady vysshei shkoly. Biologicheskie nauki","volume":" 2","pages":"33-40"},"PeriodicalIF":0.0000,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Restriction endonucleases: the detection of new producer strains and the isolation of enzyme preparations].\",\"authors\":\"L R Lebedev, I S Andreeva, G N Afinogenova, Iu P Zernov, G D Serov, V E Chizhikov, N M Pustoshilova\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The method for analysis of microorganisms for the presence of the modification-restriction systems has been developed. The method has permitted to detect more than 10 new producing strains of restrictases including microorganisms of Rhizobium genus. Some of them are promising for practical use. It has been shown that using selection of clones the strain productivity can be increased. The purification process for the majority of restrictases has been proposed. Some physical and catalytic properties of new enzymes have been studied.</p>\",\"PeriodicalId\":18920,\"journal\":{\"name\":\"Nauchnye doklady vysshei shkoly. Biologicheskie nauki\",\"volume\":\" 2\",\"pages\":\"33-40\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1992-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nauchnye doklady vysshei shkoly. Biologicheskie nauki\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nauchnye doklady vysshei shkoly. Biologicheskie nauki","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Restriction endonucleases: the detection of new producer strains and the isolation of enzyme preparations].
The method for analysis of microorganisms for the presence of the modification-restriction systems has been developed. The method has permitted to detect more than 10 new producing strains of restrictases including microorganisms of Rhizobium genus. Some of them are promising for practical use. It has been shown that using selection of clones the strain productivity can be increased. The purification process for the majority of restrictases has been proposed. Some physical and catalytic properties of new enzymes have been studied.