考虑幽门螺杆菌致病菌株的消化性胃溃疡血浆纤维蛋白溶解和蛋白溶解活性

L. M. Honcharuk, O. Fediv, V. Kulachek, Y. Teleki
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引用次数: 0

摘要

本研究的目的是在考虑致病性幽门螺杆菌(Hp)菌株的情况下,探讨消化性溃疡(PU)患者血浆纤维蛋白溶解和蛋白溶解活性的变化。材料和方法。共检查93例PU患者,其中PU合并Hp cag cag A+/vac A+ 30例(I组),PU合并Hp cag A-/vac A- 31例(II组),PU合并Hp cag A-/vac A- 32例(III组),对照组为30例健康人。在偶氮纤维蛋白(与偶氮染料橙色相关的纤维蛋白)裂解的帮助下,研究了血浆的纤维蛋白溶解活性,偶氮纤维蛋白在碱性培养基中变成明亮的红色。评估总(ТFA)、酶(FFA)和非酶溶纤维蛋白活性(NFA)水平。通过偶氮白蛋白、偶氮酪蛋白和偶氮唑的溶解测定血浆的蛋白水解活性。研究的结果。血浆纤溶活性研究显示,与对照指标相比,各组血浆总纤溶活性(TFA)均显著升高:ⅰ组提高61.5%,ⅲ组提高40.9%,ⅲ组提高30.3%,组间差异有统计学意义。TFA的增长主要是由于FFA。与对照组相比,ⅰ组患者FFA增加了2.06倍(p < 0.05),ⅱ组患者FFA增加了1.79倍(p < 0.05), IIІ组患者FFA增加了1.52倍(p < 0.05)。与ⅱ组比较,ⅰ组患者FFA增高12.5% (p < 0.05)。在所有的病人检查,有血浆蛋白水解活性的增加,特别是在我组,azoalbumin的裂解,azocasein azocolol显著增加2.94倍,2.83倍和1.90倍,分别和二组患者的调查指标相应增加1.87倍(p < 0.05), 1.96倍(p < 0.05)和1.40倍(p < 0.05),第三组患者分别1.55倍(p < 0.05), 1.59倍(p < 0.05)和1.18倍,与几乎健康的人相比。致病性Hp菌株存在时,蛋白水解的变化更为显著。PU患者血浆蛋白溶解和纤维蛋白溶解活性升高。PU中伴随Hp的存在导致蛋白溶解和纤维蛋白溶解更明显的变化。Hp cag - cag - A+/vac - A+病原菌引起的止血异常明显多于cag - cag - A+。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
FIBRINOLYTIC AND PROTEOLYTIC ACTIVITY OF BLOOD PLASMA IN PEPTIC ULCER OF THE STOMACH, TAKING INTO ACCOUNT THE PATHOGENIC STRAINS OF HELICOBACTER PYLORI
The purpose of the study is to investigate changes in fibrinolytic and proteolytic activity of blood plasma in patients with peptic ulcer (PU) taking into account pathogenic Helicobacter pylori (Hp) strains. Materials and methods. 93 patients with PU were examined, of which 30 patients with PU and concomitant Hp cag cag A+/vac A+ (group I), 31 patients with PU and concomitant Hp cag A-/vac A- (group II), 32 patients with PU without concomitant HP infection (group III). The control group consisted of 30 healthy individuals. Fibrinolytic activity of blood plasma was investigated with the help of lysis of azofibrin (fibrin associated with the azo dye orange), which in the alkaline medium turns a bright red color. The level of total (ТFA), enzymatic (FFA) and non-enzymatic fibrinolytic activity (NFA) was evaluated. Proteolytic activity of blood plasma was determined by the lysis of azoalbumin, azocasein and azokol. Research results. The study of fibrinolytic activity of blood plasma showed that the total fibrinolytic activity of blood plasma (TFA) in all groups was significantly higher compared to the control indicators: in patients of group I by 61.5 %, in patients by 40.9 %, in patients of group III by 30.3 %, with a significant intergroup difference between the groups. The growth of TFA was mainly due to FFA. In patients of group I, FFA increased by 2.06 times (p < 0.05), and in patients of group II – by 1.79 times (p < 0.05), in patients of group IIІ – by 1.52 times (p < 0.05) compared with the control. In patients with group I, FFA increased by 12.5 % ​​(p < 0.05) compared with group II. In all patients examined, there was an increase in the proteolytic activity of blood plasma, in particular in group I, the lysis of azoalbumin, azocasein and azocolol increased significantly 2.94 times, 2.83 times and 1.90 times, respectively, and in the patients of group II the investigated indicators increased accordingly 1.87-fold (p < 0.05), 1.96-fold (p < 0.05) and 1.40-fold (p < 0.05), in patients of group III, respectively 1.55 times (p < 0.05), 1.59 times (p < 0.05) and 1.18 times, compared to these values ​​in almost healthy subjects. Significantly more significant changes in proteolysis were detected in the presence of pathogenic Hp strains. Conclusion. Increased proteolytic and fibrinolytic activity of blood plasma is observed in patients with PU. The presence of concomitant Hp in PU leads to more pronounced changes in proteolysis and fibrinolysis. Pathogenic strains of Hp cag cag A+/vac A+ cause significantly more abnormalities in hemostasis.
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