N. Hassan, Wageih Armanious, Sabry Morgan, A. Samir, A. Orabi
{"title":"单核细胞增生李斯特菌在牛、山羊和绵羊乳腺感染中的生态位、抗菌敏感性和毒力研究综述","authors":"N. Hassan, Wageih Armanious, Sabry Morgan, A. Samir, A. Orabi","doi":"10.21608/vmjg.2021.192304","DOIUrl":null,"url":null,"abstract":"A total of 410 milk samples were collected from different ruminant species as the following ; cows (n = 110), buffaloes (n = 87) ) , does (n=108) ) and ewes (n=105) from different Egyptian farms .The results revealed that prominence of L.monocytogenes in the examined milk samples are 1.72 % at apparently healthy cow , 2.77 % in subclinical mastitic cow and in apparently healthy goat is 1.38 % and this has a public health importance. The recovered isolates identified biochemically as L.monocytogenes confirmed phenotypically by positive hemolytic reaction and motility , followed by molecular confirmation using specific primer sequences of 16s rRNA at 1200 bp followed with sequencing and accession on genbank . Antibiogram of the isolated L. monocytogenes are demonstrated resistance to tetracyclines, lincomycin, streptomycin, cefotaxime, Trimethoprime sulfamethoxazole, florphenicols, and clindamycin, while adherence assay for test glass tube polystyrene microtiter plates conclude that L. monocytogenes was able to form a strong biofilm.","PeriodicalId":202817,"journal":{"name":"Veterinary Medical Journal (Giza)","volume":"7 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Overview on Listeria monocytogenes ecological niches, antibacterial susceptibility and virulence in bovine, caprine and ovine udder infection as a public health implication.\",\"authors\":\"N. Hassan, Wageih Armanious, Sabry Morgan, A. Samir, A. Orabi\",\"doi\":\"10.21608/vmjg.2021.192304\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A total of 410 milk samples were collected from different ruminant species as the following ; cows (n = 110), buffaloes (n = 87) ) , does (n=108) ) and ewes (n=105) from different Egyptian farms .The results revealed that prominence of L.monocytogenes in the examined milk samples are 1.72 % at apparently healthy cow , 2.77 % in subclinical mastitic cow and in apparently healthy goat is 1.38 % and this has a public health importance. The recovered isolates identified biochemically as L.monocytogenes confirmed phenotypically by positive hemolytic reaction and motility , followed by molecular confirmation using specific primer sequences of 16s rRNA at 1200 bp followed with sequencing and accession on genbank . Antibiogram of the isolated L. monocytogenes are demonstrated resistance to tetracyclines, lincomycin, streptomycin, cefotaxime, Trimethoprime sulfamethoxazole, florphenicols, and clindamycin, while adherence assay for test glass tube polystyrene microtiter plates conclude that L. monocytogenes was able to form a strong biofilm.\",\"PeriodicalId\":202817,\"journal\":{\"name\":\"Veterinary Medical Journal (Giza)\",\"volume\":\"7 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary Medical Journal (Giza)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21608/vmjg.2021.192304\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Medical Journal (Giza)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/vmjg.2021.192304","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Overview on Listeria monocytogenes ecological niches, antibacterial susceptibility and virulence in bovine, caprine and ovine udder infection as a public health implication.
A total of 410 milk samples were collected from different ruminant species as the following ; cows (n = 110), buffaloes (n = 87) ) , does (n=108) ) and ewes (n=105) from different Egyptian farms .The results revealed that prominence of L.monocytogenes in the examined milk samples are 1.72 % at apparently healthy cow , 2.77 % in subclinical mastitic cow and in apparently healthy goat is 1.38 % and this has a public health importance. The recovered isolates identified biochemically as L.monocytogenes confirmed phenotypically by positive hemolytic reaction and motility , followed by molecular confirmation using specific primer sequences of 16s rRNA at 1200 bp followed with sequencing and accession on genbank . Antibiogram of the isolated L. monocytogenes are demonstrated resistance to tetracyclines, lincomycin, streptomycin, cefotaxime, Trimethoprime sulfamethoxazole, florphenicols, and clindamycin, while adherence assay for test glass tube polystyrene microtiter plates conclude that L. monocytogenes was able to form a strong biofilm.