发芽幼苗中氧基化合物的检测

A. Oliva-Hernández, J. D. C. Quiroz-Velásquez, J. G. García-Olivares, Israel García-León, Cristian Lizarazo-Ortega, J. L. Hernández-Mendoza
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引用次数: 0

摘要

色氨酸(TRP)是一种代谢物,在植物、动物和人类中产生几种重要的代谢合成。在细菌和真菌中,它是吲哚乙酸(IAA)的前体,通过多种代谢途径生成。本研究的目的是检测几种植物种子萌发过程中IAA合成的中间代谢物。在研究中,为了刺激种子萌发,将植物种子置于去离子水中,每24小时采集一次样本。采用高效液相色谱法对化合物进行检测。结果表明,介质的pH值发生了变化,没有行为模式。对于检测到的化合物,除了TRP外,还有吲哚-3-乙酰胺(IAM), 3-吲哚-乙腈(IAN),色胺(TRM),它们是TRP依赖途径的一部分,因为它们使用该氨基酸作为前体。此外,还检测到独立TRP通路中的邻氨基苯酸(AA)和犬尿氨酸(KYN)。在研究的种子(双色高粱、T . aesativum、玉米、Phaseolus vulgaris、G. hirsutum、Cucurbita maxima)萌发过程中也检测到IAA和TRP。最后,我们观察到种子在萌发过程中由于重量减少而遭受物理磨损,因为在研究结束时种子的初始干重和重量之间存在差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detection of auxinic compounds in germinating seedlings
Tryptophan (TRP) is a metabolite from which several important metabolic syntheses arise in plants, animals, and humans. In bacteria and fungi, it is a precursor of Indole Acetic acid (IAA) using various metabolic pathways. The objective of this study is the detection of intermediate metabolites in the synthesis of IAA in seeds of several species in the germination process. In the study, seeds of plant species grown in deionized water were placed in order to stimulate germination and samples were taken every 24 hours. High performance liquid chromatography (HPLC) was used for the detection of the compounds. The results show that the pH of the medium is altered and there is no pattern of behavior. Regarding the detected compounds, in addition to TRP, there is indole-3-acetamide (IAM), 3-indoleacetonitrile (IAN), tryptamine (TRM), which are part of the TRP-dependent routes, since they use this amino acid as a precursor. Anthranilic acid (AA) and kynurenine (KYN), which are part of the Independent TRP pathway, were also detected. IAA and TRP were also detected during the germination process of the studied seeds (Sorghum bicolor, T aesativum, Zea mayz, Phaseolus vulgaris, G. hirsutum, Cucurbita maxima). Finally, it was observed that the seeds, due to weight loss, suffer physical wear during the germination process, since there is a difference between the initial dry weight and the weight of the seeds at the end of the study.
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