Formation of Electric Fields in Optically-induced Dielectrophoresis

In this paper, the formation of electric field in optically-induced dielectrophoresis with different light patterns was simulated, which provided a theoretical basis for experiments, and the influence of frequency on the manipulation results was found through simulations and experiments. We simulated the corresponding non-uniform electric field generated by the light patterns of dot, ring and strip arrays respectively, and obtained the intuitive electric field distribution and the distribution curve of the gradient of the square of the electric field strength directly related to the light induced dielectrophoresis force, which provided a theoretical basis for the experiment. The experiments verified the accuracy of the simulation. Fromg simulations and experiments, it is found that even if the crossover frequency is not involved, the frequency change of the electrical signal can also affect the position of the cells being manipulated.