BIOEFFICACY OF Metarhizium (Nomuraea) rileyi ON PROTEIN PROFILE DURING EMBRYOGENESIS OF Helicoverpa armigera (HUBNER)

Entamopathogenic fungi, Metarhizium rileyi is one of the promising alternative over chemical insecticides for the management of a most serious polyphagus pest, H. armigera. Metarhizium rileyi was tested for their median lethal concentrations (LC50) and qualitative proteins against Helicoverpa armigera was carried out at Research laboratory, P.G. Department of Zoology, YashvantaraoChavan Institute of Science,Sataraunder Shivaji University, Kolhapur in 2019. M. rileyi is an effective ovicide, due to its ability to penetrate the eggs actively through their shell and multiply inside them. Yolk proteins are the primary nutrient resources in eggs which maintain nutritional quality to remain viable during embryogenesis. The LC50 value of M. rileyi was recorded as 3.8×102 spores/ml against eggs of H. armigera. The LC50concentration of 3.8×102 spores/ml of M. rileyi was applied to the eggs of H. armigera to investigate their impacts on the total protein. The qualitative analysis of protein patterns was done by using SDS-PAGE electrophoresis. The SDS-PAGE analysis of eggs demonstrated that the few of proteins get under regulated upon treatment with Metarhizium rileyi.