腹腔分次x射线照射后小鼠空肠上皮的增殖模式。

S Lesher, J Cooper, R Hagemann, J Lesher
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引用次数: 0

摘要

研究了腹腔分次x射线照射小鼠肠上皮细胞的增殖。暴露时间分别为12、24、48或72小时。使用隐窝挤压技术,在1、2、3、5、7和9次暴露后,以紧密间隔的时间间隔(1至96小时)测定每个隐窝的标记细胞核和有丝分裂图像。在12和24小时测定细胞周期时间,包括估计G-1、S、G-2和M。这些数据表明,肠上皮在严重损伤后,如果有足够的时间加速增殖活性,则具有显著的修复损伤和恢复的潜力。损伤-修复-恢复模式,即增殖种群规模的增加和世代周期的加速,在单次和分次暴露中是相似的。高度组织化的体内细胞群能够耐受的300r暴露的数量取决于各组分之间的时间间隔;例如,当剂量间隔为12小时时,8次300r暴露会杀死所有动物,但当间隔增加到72小时时,一些动物能存活20次剂量,尽管死亡时间变化很大。在大量的暴露中保持了补偿性恢复的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Proliferative patterns in the mouse jejunal epithelium after fractionated abdominal x-irradiation.

Cell proliferation was studied in the intestinal epithelium of mice exposed to fractionated abdominal X-irradiation. Exposures were separated by 12, 24, 48 or 72 hr. Labelled nuclei and mitotic figures per crypt using the crypt squash technique were determined at closely-spaced time intervals (1 to 96 hr) after 1, 2, 3, 5, 7 and 9 exposures. Cell cycle times including estimates of G-1, S, G-2 and M were determined at 12 and 24 hr. These data show that the intestinal epithelium has a remarkable potential to repair damage and recover following severe injury if sufficient time is allowed for the acceleration in proliferative activity. The damage-repair-recovery pattern, i.e., increase in size of proliferative population and acceleration of the generation cycle, is similar for single and fractionated exposures. The number of 300 R exposures which can be tolerated by a highly organized in vivo cell population is dependent upon time interval between fractions; e.g., when interval between fractions is 12 hr eight 300 R exposures kill all animals, but when the interval is increased to 72 hr some animals survive 20 doses, although time of death is highly variable. The compensatory recovery potential is maintained over a large number of exposures.

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