分子鉴定
引用次数: 2
摘要
............................................................................................................................... 3确认 .............................................................................................................. 4名单表 ....................................................................................................................... 7数据列表 ...................................................................................................................... 8第1章向地性 .................................................................................................... 9Gravitropic通路 ....................................................................................... 10感知 .................................................................................................. 10生长素的再分配 .................................................................................. 13响应 .................................................................................................... 16信号转导 ................................................................................... 17研究的焦点 ...................................................................................................... 22参考书目 .......................................................................................................... 25第二章描述的GPS2基因 .............................................................. 31日材料和方法 ............................................................................................. 32植物,生长条件和DNA提取 ......................... 32热不对称交错聚合酶链反应..................32克隆PCR产物 ...................................................................... 36构造 .................................................................................................. 38农杆菌转化 ................................................................. 43花和拟南芥转换 .............................................. 43本文章由计算机程序翻译，如有差异，请以英文原文为准。
Molecular Characterization
...............................................................................................................................3 Acknowledgements..............................................................................................................4 List of Tables .......................................................................................................................7 List of Figures ......................................................................................................................8 Chapter 1 Gravitropism....................................................................................................9 The Gravitropic Pathway .......................................................................................10 Perception ..................................................................................................10 Auxin Redistribution..................................................................................13 Response ....................................................................................................16 Signal Transduction ...................................................................................17 Research Focus ......................................................................................................22 Bibliography ..........................................................................................................25 Chapter 2 Characterization of the GPS2 Gene ..............................................................31 Materials and Methods.............................................................................................32 Plant Material, Growth Conditions, and DNA Extraction.........................32 Thermal Asymmetric Interlaced Polymerase Chain Reaction ..................32 Cloning of the PCR Product ......................................................................36 Constructs ..................................................................................................38 Agrobacterium Transformation .................................................................43 Floral Dip and Arabidopsis Transformation ..............................................43
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