光学法测定光散射介质中发光团的含量

I. Raznitsyna, D. Rogatkin, A. Tarasov
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引用次数: 0

摘要

本文研究了用激光荧光光谱法和漫反射光谱法测定浑浊介质中发光团相对浓度的问题。提出的方法是基于改进的Kubelka-Munk理论模型,并使用从浑浊生物幻影介质中获得的实验数据。研究了散射对探测器记录的辐射的影响,并给出了校正曲线。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Determination of Luminophores Content in Light-Scattering Phantom Medium by Optical Methods
The paper considers a problem of determination of the luminophore relative concentration in a turbid medium using a laser fluorescence spectroscopy and a diffuse reflectance spectroscopy. The proposed approach is based on the improved Kubelka-Munk theoretical model with the use of experimental data obtained from turbid biological phantom media. Influence of scattering on the radiation registered by a detector is also investigated providing calibration curves to solve the problem.
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